Product Name:

PYK


Product Number:

ab-np049-1

Price:

Regular price
$89.00
Regular price
Sale price
$89.00

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Product Type Specific: Generic phosphotyrosine-specific antibody

Antibody Code: PG001

Antibody Target Type: Generic phosphotyrosine-specific

Antibody Phosphosite: Generic phospho

Antibody Type: Polyclonal

Antibody Host Species: Rabbit

Antibody Immunogen Source: Over 200 diverse phosphotyrosine phosphorylation site peptides

Antibody Immunogen Description: Diverse locations

Production Method: Diverse locations

Antibody Modification: Generic phosphotyrosine-specific antibody

Antibody Concentration: 1 mg/ml

Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol

Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.

Product Use: Western blotting | Antibody microarray

Antibody Dilution Recommended: 2 µg/ml for immunoblotting

Antibody Species Reactivity: Human

Table 1. Immunoreactivities of generic phosphotyrosine-specific antibodies with the 6099 tyrosine-phosphorylated peptides on the Jerini JPT Phosphatase Peptide Microarray and ~500 phosphopeptides on the Kinex™ (KPSM) Phosphopeptide Microarray.

Figure 1. Phosphoamino acid specificity of ~2 µg/ml PYK, ~5 µg/ml 4G10, ~5 µg/ml PY-20, and ~1 µg/ml PY-100 with 500 phosphopeptides on the Kinex™ (KPSM) Phosphopeptide Microarray. The peptide signals (normalized to median value) for each antibody and type of phosphorylated peptide were sorted in order of the strongest signal first.

Figure 2. Phosphotyrosine peptide reactivity of ~2 µg/ml PYK, ~5 µg/ml 4G10, ~5 µg/ml PY-20, and ~1 µg/ml PY-100 for 6099 phosphotyrosine-containing peptides on the Jerini JPT Phosphatase Peptide Microarray. The peptide signals (normalized to median value) for each antibody were sorted in order of the strongest signal first.