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Product Type Specific: Generic phosphotyrosine-specific antibody
Antibody Code: PG001
Antibody Target Type: Generic phosphotyrosine-specific
Antibody Phosphosite: Generic phospho
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Over 200 diverse phosphotyrosine phosphorylation site peptides
Antibody Immunogen Description: Diverse locations
Production Method: Diverse locations
Antibody Modification: Generic phosphotyrosine-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Related Product 1: PYK-Ultra generic phosphotyrosine-specific antibody (Cat. No.: AB-PG001U)
Related Product 2: Phosphotyrosine pan-specific antibody (Cat. No.: AB-CN005-2)
Related Product 3: PYKSD8 generic phospho-specific antibody (Cat. No.: AB-PG005)
Table 1. Immunoreactivities of generic phosphotyrosine-specific antibodies with the 6099 tyrosine-phosphorylated peptides on the Jerini JPT Phosphatase Peptide Microarray and ~500 phosphopeptides on the Kinex™ (KPSM) Phosphopeptide Microarray.
Figure 1. Phosphoamino acid specificity of ~2 µg/ml PYK, ~5 µg/ml 4G10, ~5 µg/ml PY-20, and ~1 µg/ml PY-100 with 500 phosphopeptides on the Kinex™ (KPSM) Phosphopeptide Microarray. The peptide signals (normalized to median value) for each antibody and type of phosphorylated peptide were sorted in order of the strongest signal first.
Figure 2. Phosphotyrosine peptide reactivity of ~2 µg/ml PYK, ~5 µg/ml 4G10, ~5 µg/ml PY-20, and ~1 µg/ml PY-100 for 6099 phosphotyrosine-containing peptides on the Jerini JPT Phosphatase Peptide Microarray. The peptide signals (normalized to median value) for each antibody were sorted in order of the strongest signal first.