Product Name: MKK3 (215-221) pS218
Product Number: PE-04BAB00
| Size: | 200 µg | Price: | 22.00 | |
| 1 mg | $US | 44.00 |
Peptide Name: MKK3 (215-221) pS218
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases and for epitope mapping of phosphosite-specific antibodies. The peptide sequence is located in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. S218 phosphorylation stimulates phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: LVD-pS-VAK
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Modifications Other: Phosphorylated
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases and for epitope mapping of phosphosite-specific antibodies. The peptide sequence is located in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. S218 phosphorylation stimulates phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: LVD-pS-VAK
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 809.83 Da
Peptide Purity Percent after Synthesis and Purification: >70
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Purity Percent after Synthesis and Purification: >70
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Scientific Background: MKK3 (MAP2K3, MEK3) is a Dual specificity protein kinase of the STE group and STE7 family. MKK3 is activated by cytokines and environmental stress in vivo. Phosphorylation of S218 and T222 increases its phosphotransferase activity. S218A and T222A substitutions are involved in inactivation. S218E and T222E mutations are associated in constitutive activation. It catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the activation T loops of the p38 MAP kinase isoforms. Defects in the MKK3 gene may be involved colon cancer.
© Kinexus Bioinformatics Corporation 2017