Product Name: ROCK1 (229-243) pT233+pT237+pT240
Product Number: PE-04AXE90
| Size: | 200 µg | Price: | 24.00 | |
| 1 mg | $US | 49.00 |
Peptide Name: ROCK1 (229-243) pT233+pT237+pT240
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. T233 phosphorylation is predicted to stimulate phosphotransferase activity. Phosphorylation of T237 and T240 are predicted to be inhibitory. These putative phosphosites are located in the kinase activation loop between catalytic subdomains VII and VIII.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: VRCD-pT-AVG-pT-PD-pY-ISP
Peptide Modifications N Terminus: Free amino
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. T233 phosphorylation is predicted to stimulate phosphotransferase activity. Phosphorylation of T237 and T240 are predicted to be inhibitory. These putative phosphosites are located in the kinase activation loop between catalytic subdomains VII and VIII.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: VRCD-pT-AVG-pT-PD-pY-ISP
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 2048.9 Da
Peptide Purity Percent after Synthesis and Purification: <50
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Storage Stability: Not stable
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 2048.9 Da
Peptide Purity Percent after Synthesis and Purification: <50
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Storage Stability: Not stable
Scientific Background: ROCK1 (ROK-beta) is a protein-serine/threonine kinase of the AGC group and DMPK family. It is a key regulator of actin cytoskeleton and cell polarity. It is activated by binding the small G protein RhoA. D1113A substitution leads to abolished cleavage by caspase-3. Down-regulating ROCK1 may be useful strategy for inhibiting migration and invasion of glioma and gastric cancer.
© Kinexus Bioinformatics Corporation 2017