Product Name: Ret (897-908) pY900+pY905
Product Number: PE-04AXD75
| Size: | 200 µg | Price: | 42.00 | |
| 1 mg | $US | 84.00 | ||
| 5 mg | 191.00 |
Peptide Name: Ret (897-908) pY900+pY905
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases. The presentation as a dimer increases the potential activity. The peptide sequence is located in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. Y900 and Y905 phosphorylation stimulate phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: (RDV-pY-EEDS-pY-VKR-βA-Cys)2
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases. The presentation as a dimer increases the potential activity. The peptide sequence is located in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. Y900 and Y905 phosphorylation stimulate phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: (RDV-pY-EEDS-pY-VKR-βA-Cys)2
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Modifications Other: Phosphorylated, dimer
Peptide Molecular Mass Calculated: 3865.6 Da
Peptide Purity Percent after Synthesis and Purification: ~50
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Storage Stability: Stable as powder; solution tends to dimerize
Peptide Molecular Mass Calculated: 3865.6 Da
Peptide Purity Percent after Synthesis and Purification: ~50
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Storage Stability: Stable as powder; solution tends to dimerize
Scientific Background: Ret is a receptor protein-tyrosine in the TK group of protein kinases in the Ret family. It is highly expressed and widely distributed in most tested human tissues. Ret is activated by binding one of four structurally ligands (i.e. GDNF, neurturin, artemin and persephin). Autophosphorylation of S696, Y806, Y809, Y864, Y900, Y905, Y928, Y952, Y981 and Y1062 of Ret increases its phosphotransferase activity. Phosphorylation of Y1015 inhibits its phosphotransferase activity. Protein interactions are induced with phosphorylation of Y981 (with Src), Y1062 (with Crk, Dok6, FRS2, Gab1, IRS1, PIK3R1, RapGEF1, Shc1 & Shc3), and Y1096 (with Grb2 & PIK3R1). Phosphorylation of Y1015 inhibits kinase activity. Ret orchestrates intestine organogenesis. Germline mutations of Ret cause a dominantly inherited dysgenesis of the enteric nervous system known as Hirschsprung's disease. Ret can undergo oncogenic activation by cytogenetic rearrangement. It is constitutively activated by point mutations in hereditary medullary thyroid carcinomas (MTCs). Multiple endocrine neoplasia type 2A (MEN 2A) is associated with two mutations of Ret. It was identified as a potential low-penetrance gene for apparently sporadic pheochromocytoma. Ret has also been linked with congenital central hypoventilation syndrome, bladder carcinomas, and colorectal adenocarcinomas.
© Kinexus Bioinformatics Corporation 2017