Product Name: NLK (302-309) pT303+pY306
Product Number: PE-04AWS99
| Size: | 200 µg | Price: | 16.00 | |
| 1 mg | $US | 31.00 | ||
| 5 mg | 85.00 |
Peptide Name: NLK (302-309) pT303+pY306
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. T303 and Y306 phosphorylation are predicted to be inhibitory for phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: V-pT-QY-pY-RAP
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. T303 and Y306 phosphorylation are predicted to be inhibitory for phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: V-pT-QY-pY-RAP
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 1372.3 Da
Peptide Purity Percent after Synthesis and Purification: <50
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Storage Stability: solution tends to dimerize
Peptide Purity Percent after Synthesis and Purification: <50
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Storage Stability: solution tends to dimerize
Scientific Background: NLK is a protein-serine/threonine kinase that is a member of the CMGC group of protein kinases in the MAPK family, and nmo subfamily. This kinase is highly expressed and widely distributed in most tested human tissues. NLK is activated by phosphorylation at tyrosine and threonine, and by by activin. NLK plays a role in cell fate determination and is required for the differentiation of bone marrow stromal cells. NLK also suppresses a wide range of transcription factors such as CREB by phosphorylation of the co-activator CREBBP. NLK expression increases the number of apoptotic cells but does not induce obvious cell cycle arrest. NLK suppresses Notch signalling by interfering with formation of the Notch active transcriptional complex. NLK has been linked with the development of glioblastoma multiforme (GM). Overexpression of NLK is associated with suppressed human colon cancer cell line growth. NLK inhibits human adenosquamous carcinoma cells proliferation and block cell cycle progression to S phase.
© Kinexus Bioinformatics Corporation 2017