Product Name: MELK (159-170) pY163+pT167
Product Number: PE-04AWJ90
| Size: | 200 µg | Price: | 124.00 | |
| 1 mg | $US | 248.00 |
Peptide Name: MELK (159-170) pY163+pT167
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. T167 phosphorylation stimulates phosphotransferase activity. Effect of Y163 phosphorylation is unknown, but likely to be stimulatory.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: GNKD-pY-HLQ-pT-CCG-pS-LA-pY-AAP
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. T167 phosphorylation stimulates phosphotransferase activity. Effect of Y163 phosphorylation is unknown, but likely to be stimulatory.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: GNKD-pY-HLQ-pT-CCG-pS-LA-pY-AAP
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 2547.6 Da
Peptide Purity Percent after Synthesis and Purification: >90
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Molecular Mass Calculated: 2547.6 Da
Peptide Purity Percent after Synthesis and Purification: >90
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Scientific Background: MELK is a protein-serine/threonine kinase of the CAMK group and CAMKL family. It can occur in 8 human isoforms ranging from 74.642 to 52.528 kDa in size. It is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It is activated by autophosphorylation of the T-loop at T167 and S171: in contrast to other members of the SNF1 subfamily, phosphorylation at T167 is not mediated by STK11/LKB1 but via autophosphorylation instead. Phosphorylation at T478 induces interaction with NIPP-1. It exhibits a broad substrate specificity with targets that include BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. It is an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1, and its phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. It is required for proliferation of embryonic and postnatal multipotent neural progenitors. It phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of its pro-apoptotic function. It inhibits spliceosome assembly during mitosis by phosphorylating ZNF622, which contributes to its redirection to the nucleus. It interacts with ZNF622 and PPP1R8. It is expressed in placenta, kidney, thymus, testis, ovary and intestine, and is up-regulated in many cancers cells.
© Kinexus Bioinformatics Corporation 2017