Product Name: MAPKAPK5 (179-192) pT182+pT186+pT190+pY192+pY193
Product Number: PE-04AWG80
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Peptide Name: MAPKAPK5 (179-192) pT182+pT186+pT190+pY192+pY193
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. T182 phosphorylation is stimulatory for phosphotransferase activity and T186 phosphorylation is predicted to be activatory. T190, Y192 and Y193 phosphorylations are predicted to be inhibitory for phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: DLM-pT-PQF-pT-P-pY-pY-VAP
Peptide Modifications N Terminus: Free amino
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. T182 phosphorylation is stimulatory for phosphotransferase activity and T186 phosphorylation is predicted to be activatory. T190, Y192 and Y193 phosphorylations are predicted to be inhibitory for phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: DLM-pT-PQF-pT-P-pY-pY-VAP
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 2178.1 Da
Peptide Purity Percent after Synthesis and Purification: <50
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 2178.1 Da
Peptide Purity Percent after Synthesis and Purification: <50
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Scientific Background: MAPKAPK5 is a protein-serine/threonine kinase of the CAMK group and MAPKAPK family. p38 alpha and beta-dependent phosphorylation increases its activity. It is activated by stress-related extracellular stimuli; such as H2O2, arsenite, anisomycin TNF alpha and also PMA and the calcium ionophore A23187; but to a lesser extent. In vitro, activated by SQSTM1. T182A substitution leads to no p38-beta/MAPK11-induced activation. In addition, T182D, S212D and L337G mutations are associated with higher protein kinase activity. It appears to be a tumour suppressor protein (TSP) that mediates Ras-induced senescence and phosphorylates p53/TP53. It also phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, and RHEB.
© Kinexus Bioinformatics Corporation 2017