Product Name: MAPKAPK2 (219-232) pT222+pY225+pT226+pY228+pY229
Product Number: PE-04AWF95
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Peptide Name: MAPKAPK2 (219-232) pT222+pY225+pT226+pY228+pY229
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. T222 and Y225 phosphorylation stimulates phosphotransferase activity. T226, Y228 and Y229 phosphorylation are predicted to be inhibitory.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: SLT-pT-PC-pY-pT-P-pY-pY-VAP
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. T222 and Y225 phosphorylation stimulates phosphotransferase activity. T226, Y228 and Y229 phosphorylation are predicted to be inhibitory.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: SLT-pT-PC-pY-pT-P-pY-pY-VAP
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 2191 Da
Peptide Purity Percent after Synthesis and Purification: <50
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Molecular Mass Calculated: 2191 Da
Peptide Purity Percent after Synthesis and Purification: <50
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Scientific Background: MAPKAPK2 is a protein-serine/threonine kinase that is a member of the CAMK group of protein kinases in the MAPKAPK family, and MAPKAPK subfamily. This kinase is highly expressed and widely distributed in most tested human tissues. It functions in the regulation of various cellular processes, including cytokine production, endocytosis, cytoskeletal reorganization, cell migration, cell cycle control, chromatin remodelling, response to DNA damage, and transcription. Phosphorylation of T222, T334 and S272 increases its phosphotransferase activity. It seems to be activated by two distinct pathways: the first involves the stimulation of p42/p44 MAPK by growth factors, the second, triggered by stress and heat shock, depends on the activation of MPK2 and upstream MAPKK/MAPKKK. It mediates both ERK and p38 MAPK-dependent neutrophil responses, participates in TNFa-stimulated exocytosis of secretory vesicles in neutrophils, and plays a role in phagocytosis-induced respiratory burst activity. Another one of its major substrates is HSP27, a protein responsble for stimulating actin polymerization in order to facilitate the recovery from cytoskeleton destruction during cellular stress. Over-expression of the MAPKAPK2 gene has been linked to Multiple Myeloma resistance to chemotherapy, and it has been found to play a role in neuroinflammatory and neurodegenerative diseases such as Alzheimer's. MAPKAPK2 may be a tumour requiring protein (TRP). It has also been implicated in the promotion of cancer development and tumourigenesis, specifically lung cancer.
© Kinexus Bioinformatics Corporation 2017