Product Name: EIF2AK1 (483-490) pT486+pT488+pS489
Product Number: PE-04AUW01
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Peptide Name: EIF2AK1 (483-490) pT486+pT488+pS489
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. T488 phosphorylation stimulates phosphotransferase activity, whereas T486 and S489 phosphorylation are also predicted to be activatory.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: RTP(pT)H(pT)(pS)R
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. T488 phosphorylation stimulates phosphotransferase activity, whereas T486 and S489 phosphorylation are also predicted to be activatory.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: RTP(pT)H(pT)(pS)R
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Modifications Other: Phosphorylated
Storage Conditions: -20°C
Storage Conditions: -20°C
Scientific Background: (HRI) EIF2AK1 is a protein-serine/threonine kinase in the Other group, and PEK family. It inhibits protein synthesis at the translation initiation level, in response to diverse stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock.HRI exerts its function through the phosphorylation of EIF2S1 at S48 and S51, and preventing its recycling. It binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. With this unique heme-sensing capacity, HRI plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Consequently, it plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. It may also contain ER stress during acute heme-deficient conditions
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