Product Name: GCK (161-173) pS164+pS170
Product Number: PE-04AUN90
| Size: | 200 µg | Price: | 40.00 | |
| 1 mg | $US | 81.00 |
Peptide Name: GCK (161-173) pS164+pS170
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases. The presentation as a dimer increases the potential activity. The peptide sequence is located in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. S170 phosphorylation stimulates phosphotransferase activity, whereas S164 phosphorylation is predicted to also be activatory.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: (LTA-pS-VAKRR-pS-FIG-βA-Cys)2
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases. The presentation as a dimer increases the potential activity. The peptide sequence is located in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. S170 phosphorylation stimulates phosphotransferase activity, whereas S164 phosphorylation is predicted to also be activatory.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: (LTA-pS-VAKRR-pS-FIG-βA-Cys)2
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Modifications Other: Phosphorylated, dimer
Peptide Molecular Mass Calculated: 3560.2 Da
Peptide Purity Percent after Synthesis and Purification: >50
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Storage Stability: Stable as dimer
Peptide Molecular Mass Calculated: 3560.2 Da
Peptide Purity Percent after Synthesis and Purification: >50
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Storage Stability: Stable as dimer
Scientific Background: GCK (MAP4K2) is a protein-serine/threonine kinase of the STE group and STE20 family. It participates in the regulation of the MAPK signalling pathway. It acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signalling pathway and to a lesser extend of the p38 MAPKs signalling pathway. It is required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin. IL-1 and engagement of CD40 also stimulate to a lessor degree GCK-mediated JNKs activation. GCK is important for LPS stimulation of c-Jun phosphorylation and induction of IL-8. It enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. It mediates the JNK signalling pathway and the p38 MAPKs signalling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3. It may play a role in the regulation of vesicle targeting or fusion. regulation of vesicle targeting or fusion. It has been linked to insulinomas, which are tumours of the pancreas arising from beta cells, and which will secrete insulin.
© Kinexus Bioinformatics Corporation 2017