Product Name: CLK1 (334-348) pS337+pT338+pS341+pT342+pY345
Product Number: PE-04ASV90
| Size: | 200 µg | Price: | 31.00 | |
| 1 mg | $US | 63.00 | ||
| 5 mg | 171.00 |
Peptide Name: CLK1 (334-348) pS337+pT338+pS341+pT342+pY345
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. Phosphorylation of S337, and T338 are predicted to stimulate phosphotransferase activity, whereas phosphorylation of S341, T342 and Y345 are predicted to be inhibitory.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: EHH-pS-pT-LV-pS-pT-RH-pY-RAP
Peptide Modifications N Terminus: Free amino
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. Phosphorylation of S337, and T338 are predicted to stimulate phosphotransferase activity, whereas phosphorylation of S341, T342 and Y345 are predicted to be inhibitory.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: EHH-pS-pT-LV-pS-pT-RH-pY-RAP
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 2364.1 Da
Peptide Purity Percent after Synthesis and Purification: >50
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Storage Stability: Not stable
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 2364.1 Da
Peptide Purity Percent after Synthesis and Purification: >50
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Storage Stability: Not stable
Scientific Background: CLK1 is a protein-serine/threonine kinase of the CMGC group and CLK family. It is a dual-specificity kinase that phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may involve in regulatory mechanisms of RNA splicing. The phosphorylated serine/arginine-rich (SR) proteins involves in the pre-mRNA processing and releasing through nucleus into nucleoplasm. CLK1, which could phosphorylates the specific SR proteins such as ASF/SF2, may directly regulates the activity and compartmentalization of SR splicing factors. CLK1 interacts with PPIG. This kinase shows high variability in human tissue distribution with the highest levels detected in adrenals, brain, kidney, lung, ovary, pituitary, prostate, spinal cord, testes, thymus, thyroid, trachea, and uterus. Orthologues of CLK1 are amongst the most highly conserved protein kinases in animals, plants, fungi and unicellular eukaryotes.
© Kinexus Bioinformatics Corporation 2017