Product Name: PKCz (258-267) pS262+pY263
Product Number: PE-04ARQ99
| Size: | 200 µg | Price: | 76.00 | |
| 1 mg | $US | 152.00 | ||
| 5 mg | 332.00 |
Peptide Name: PKCz (258-267) pS262+pY263
Product Use: Services as a blocking peptide for use with the PKCz-pS262+Y263 rabbit polyclonal antibody (Cat. No.: AB-PK774) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the kinase catalytic subdomain I region. S262 and Y263 phosphorylation is predicted to be inhibitory for phosphotransferase activity. Phosphorylation of S262 induces interaction with 14-3-3 proteins.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: IGRG-pS-pY-AKVL
Peptide Modifications N Terminus: Free amino
Product Use: Services as a blocking peptide for use with the PKCz-pS262+Y263 rabbit polyclonal antibody (Cat. No.: AB-PK774) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the kinase catalytic subdomain I region. S262 and Y263 phosphorylation is predicted to be inhibitory for phosphotransferase activity. Phosphorylation of S262 induces interaction with 14-3-3 proteins.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: IGRG-pS-pY-AKVL
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 1438.48 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: PKCz - pS262+pY263 phosphosite-specific antibody (Cat. No.: AB-PK774)
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 1438.48 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: PKCz - pS262+pY263 phosphosite-specific antibody (Cat. No.: AB-PK774)
Scientific Background: PKCz is one of several protein kinases that can phosphorylate KinSub1RRDSG. Human PKCz is a protein-serine/threonine kinase of 592 amino acid length, with a predicted molecular mass of 67,660 Da. It is a member of the AGC group of protein kinases in the PKC family, and Iota subfamily. It is moderate to highly expressed in most tested human tissues. Orthologues are highly conserved in vertebrates, including amphibians. PI 3,4,5-trisphosphate activates PKCz. Phosphorylation at Y356, T410 and T560 increases PKCz kinase activity. Phosphorylation of Y356 induces interaction with 14-3-3 to increase PKCz kinase activity and facilitate its complex formation with Raf1 to activate it. Phosphorylation of S113, S148, T179, S186, S217, S218, S262, and S520 induces interaction with 14-3-3 beta and Raf1. PKCz is found in both particulate and soluble fractions and is not activated by phorbol ester. However, overexpression of PKCz and subsequent phorbol ester treatment abolished phorbol ester-induced reduction in cell proliferation (1). Overexpression of PKCz also potentiates phorbol ester-induced mitogen-activated protein (MAP) kinase activation in a PKC-dependent manner. PKCz is an upstream modulator of p70S6K, an important regulator of cell proliferation (2). PKCz has been linked with the development of colorectal adenocarcinomas.
© Kinexus Bioinformatics Corporation 2017