Product Name: KIT (818-827) pS821+pY823
Product Number: PE-04ARM95
| Size: | 200 µg | Price: | 66.00 | |
| 1 mg | $US | 133.00 | ||
| 5 mg | 313.00 |
Peptide Name: KIT (818-827) pS821+pY823
Product Use: Services as a blocking peptide for use with the Kit -S821+Y823 rabbit polyclonal antibody (Cat. No.: AB-PK674) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. S821 and Y823 phosphorylation stimulate phosphotransferase activity. Y823 phosphorylation also induces interaction with Grb2 and Grb7.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: KND-pS-N-pY-VVKG
Peptide Modifications N Terminus: Free amino
Product Use: Services as a blocking peptide for use with the Kit -S821+Y823 rabbit polyclonal antibody (Cat. No.: AB-PK674) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. S821 and Y823 phosphorylation stimulate phosphotransferase activity. Y823 phosphorylation also induces interaction with Grb2 and Grb7.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: KND-pS-N-pY-VVKG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 1498.5 Da
Peptide Purity Percent after Synthesis and Purification: >90
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: KIT - pS821+pY823 phosphosite-specific antibody (Cat. No.: AB-PK674)
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 1498.5 Da
Peptide Purity Percent after Synthesis and Purification: >90
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: KIT - pS821+pY823 phosphosite-specific antibody (Cat. No.: AB-PK674)
Scientific Background: Kit (SCFR) is a protein-tyrosine kinase of the TK group and PDGFR family. It is a receptor kinase that binds SCF/KitLG. Mast,stem cell growth factor (steel factor) binding induces Kit dimerization and autophosphorylation. Protein interactions are induced with phosphorylation at Y568 (with APS, Cbl, SHP2, & Src), Y900 (with Crk & PIK3C2A) and Y936 (with APS, Cbl, Grb2 & Grb7). Phosphorylation of S741 and S746 inhibits the kinase activity of Kit. Kit can mediate cell proliferation, survival, hematopoiesis, gametogenesis, stem cell maintenance, mast cell development, function, and migration, as well as melanogenesis. Some substrates that Kit can phosphorylate include PIK3R1, PLCG1, SH2B2/APS and CBL.This kinase is highly expressed and widely distributed in most tested human tissues. It was first identified as the cellular homologue of the feline sarcoma viral oncogene v-kit. Mutations in Kit are associated with gastrointestinal stromal tumors (GIST), mast cell leukemia, acute myelogenous leukemia (AML), and piebaldism. Kit has also been linked with the development of colorectal adenocarcinomas and melanoma progression. Insertional mutagenesis studies in mice also support a role for this protein kinase in mouse cancer oncogenesis.
© Kinexus Bioinformatics Corporation 2017