Product Name: MSK2 (191-199) pT194+pS196
Product Number: PE-04AQX85
| Size: | 200 µg | Price: | 48.00 | |
| 1 mg | $US | 96.00 |
Peptide Name: MSK2 (191-199) pT194+pS196
Product Use: Services as a blocking peptide for use with the MSK2-pT194 + pS196 rabbit polyclonal antibody (Cat. No.: AB-PK868) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. T194 and S196 phosphorylation stimulates phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: KER-pT-F-pS-FC
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Gly-Cys
Product Use: Services as a blocking peptide for use with the MSK2-pT194 + pS196 rabbit polyclonal antibody (Cat. No.: AB-PK868) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. T194 and S196 phosphorylation stimulates phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: KER-pT-F-pS-FC
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Gly-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 1336.31 Da
Peptide Purity Percent after Synthesis and Purification: >80
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: MSK2 - pT194+pS196 phosphosite-specific antibody (Cat. No.: AB-PK868)
Peptide Molecular Mass Calculated: 1336.31 Da
Peptide Purity Percent after Synthesis and Purification: >80
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: MSK2 - pT194+pS196 phosphosite-specific antibody (Cat. No.: AB-PK868)
Scientific Background: MSK2 (RPS6KA4) is a protein-serine/threonine kinase of the AGC group and RSK family. It is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. It phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. It plays an essential role in the control of RELA transcriptional activity in response to TNF. It phosphorylates S10 as well as S28 of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. It mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. It appears to induce activation of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors. F709A substitution is associated with strongly elevated basal activity. MSK2 is activated by phosphorylation at S196, S343, S347, T360, T568 and T687. Aberrant activation of members of the family have found to be linked with many human diseases, such as breast and prostate cancers.
© Kinexus Bioinformatics Corporation 2017