Product Name: WEE1 (639-645) pS642
Product Number: PE-04AQN99
| Size: | 200 µg | Price: | 42.00 | |
| 1 mg | $US | 84.00 | ||
| 5 mg | 185.00 |
Peptide Name: WEE1 (639-645) pS642
Product Use: Services as a blocking peptide for use with the Wee1-pS642 rabbit polyclonal antibody (Cat. No.: AB-PK854) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the C-terminus of the kinase. Phosphorylation inhibits phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: RSV-pS-LTI
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Product Use: Services as a blocking peptide for use with the Wee1-pS642 rabbit polyclonal antibody (Cat. No.: AB-PK854) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the C-terminus of the kinase. Phosphorylation inhibits phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: RSV-pS-LTI
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 1028.12 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: WEE1 - pS642 phosphosite-specific antibody (Cat. No.: AB-PK854)
Peptide Molecular Mass Calculated: 1028.12 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: WEE1 - pS642 phosphosite-specific antibody (Cat. No.: AB-PK854)
Scientific Background: Wee1 is a Dual specificity protein kinase of the Other group and WEE family. It is a negative regulator to entry into G2-M transition in mitosis through phosphorylation of CDK1. Wee1 is a nuclear protein that catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. Phosphorylatio of S121 induces proteolysis through interaction with BTRC and FBW1B. Phosphorylation of S53 and S123 beta-transducin repeat-containing protein recognition for degradation by ubiquitination. Phosphorylation of T187, T190 and S193 induces proteolysis through interaction with BTRC and FBW1B. Phosphorylation of S642 inhibits phosphotransferase activity and induces interaction with 14-3-3 theta. Wee1 is highly expressed in testis. Wee1 kinase phosphorylates the p34 (CDC2) -cyclin B complex on Y15 and inactivates the p34 (CDC2)-cyclin B kinase. Wee1 is a target for cancer therapy. Specific inhibition of Wee1 can be used to increase the sensitivity of T-cell leukemia and pancreatic cancer. Wee1 was found to be a novel independent prognostic marker of poor survival ovarian carcinomas.
© Kinexus Bioinformatics Corporation 2017