Product Name: PKCt (692-698) pS695
Product Number: PE-04AOX99
| Size: | 200 µg | Price: | 42.00 | |
| 1 mg | $US | 84.00 | ||
| 5 mg | 185.00 |
Peptide Name: PKCt (692-698) pS695
Product Use: Services as a blocking peptide for use with the PKCq-pS695 rabbit polyclonal antibody (Cat. No.: AB-PK772) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the Pkinase_C domain near C-terminus of the kinase. S695 phosphorylation stimulates phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: RNF-pS-FMN
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Product Use: Services as a blocking peptide for use with the PKCq-pS695 rabbit polyclonal antibody (Cat. No.: AB-PK772) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the Pkinase_C domain near C-terminus of the kinase. S695 phosphorylation stimulates phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: RNF-pS-FMN
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 1168.25 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: PKCt - pS695 phosphosite-specific antibody (Cat. No.: AB-PK772)
Peptide Molecular Mass Calculated: 1168.25 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: PKCt - pS695 phosphosite-specific antibody (Cat. No.: AB-PK772)
Scientific Background: PKCt (PKCq, PRKCT, PKC-theta) is a protein-serine/threonine kinase of the AGC group and novel PKC family. It is dependent on acidic phospholipids (e.g. phosphatidylserine) and diacylglycerol for full phosphotransferase activity, and does not require calcium. It is essential in TCR signalling for T-cell activation, differentiation, proliferation, and survival. These T-cell responses are mediated via Jun, NF-kappa-B, NFATC1 and NFATC2. The phosphotransferase activity of PKCt is unaffected by an Y90F mutation, but T-cell proliferation is inhibited. An A148E mutation results in constitutive activation of PKCt. A T219A mutation again will not inhibit kinase phosphotransferase activity, but it will inhibit localization to the plasma membrane and inhibit transactivation of IL2 promoter. Phosphorylation at Y90, T538 (activation loop of the kinase domain) and S676 (turn motif) increases phosphotransferase activity. Phosphorylation at S695 (hydrophobic region) increases phosphotransferase activity and interaction with PDK1. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis. PKCt is linked to Gastrointestinal Stromal Tumours (GANT, GIST), which are rare sarcomas arising from tissues lining the gastrointestinal tract and typically arise from the stomach, intestines, or colon. GANT has been related to neurofibromatosis and adenocarcinoma as well.
© Kinexus Bioinformatics Corporation 2017