Product Name: MELK (435-441) pY438
Product Number: PE-04AOA01
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Peptide Name: MELK (435-441) pY438
Product Use: Services as a blocking peptide for use with the MELK-pY438 rabbit polyclonal antibody (Cat. No.: AB-PK701) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the C-terminal third of the protein kinase. This is the major in vivo phosphorylation site in MELK. The effect of its phosphorylation is unclear.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: NEE-pY-FMF
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Product Use: Services as a blocking peptide for use with the MELK-pY438 rabbit polyclonal antibody (Cat. No.: AB-PK701) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the C-terminal third of the protein kinase. This is the major in vivo phosphorylation site in MELK. The effect of its phosphorylation is unclear.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: NEE-pY-FMF
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 1232.27 Da
Storage Conditions: -20°C
Related Product 1: MELK - pY438 phosphosite-specific antibody (Cat. No.: AB-PK701)
Peptide Molecular Mass Calculated: 1232.27 Da
Storage Conditions: -20°C
Related Product 1: MELK - pY438 phosphosite-specific antibody (Cat. No.: AB-PK701)
Scientific Background: MELK is a protein-serine/threonine kinase of the CAMK group and CAMKL family. It can occur in 8 human isoforms ranging from 74.642 to 52.528 kDa in size. It is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It is activated by autophosphorylation of the T-loop at T167 and S171: in contrast to other members of the SNF1 subfamily, phosphorylation at T167 is not mediated by STK11/LKB1 but via autophosphorylation instead. Phosphorylation at T478 induces interaction with NIPP-1. It exhibits a broad substrate specificity with targets that include BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. It is an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1, and its phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. It is required for proliferation of embryonic and postnatal multipotent neural progenitors. It phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of its pro-apoptotic function. It inhibits spliceosome assembly during mitosis by phosphorylating ZNF622, which contributes to its redirection to the nucleus. It interacts with ZNF622 and PPP1R8. It is expressed in placenta, kidney, thymus, testis, ovary and intestine, and is up-regulated in many cancers cells.
© Kinexus Bioinformatics Corporation 2017