Product Name: MARK2 (211-216) pT215
Product Number: PE-04ANQ95
| Size: | 200 µg | Price: | 42.00 | |
| 1 mg | $US | 84.00 | ||
| 5 mg | 185.00 |
Peptide Name: MARK2 (211-216) pT215
Product Use: Services as a blocking peptide for use with the MARK1-pT215 rabbit polyclonal antibody (Cat. No.: AB-PK695) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. This is the major in vivo phosphorylation site in MARK1. T215 phosphorylation stimulates phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: KLD-pT-FCG
Peptide Modifications N Terminus: Free amino
Product Use: Services as a blocking peptide for use with the MARK1-pT215 rabbit polyclonal antibody (Cat. No.: AB-PK695) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. This is the major in vivo phosphorylation site in MARK1. T215 phosphorylation stimulates phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: KLD-pT-FCG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 1036.1 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: MARK2 - pT215 phosphosite-specific antibody (Cat. No.: AB-PK695)
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 1036.1 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: MARK2 - pT215 phosphosite-specific antibody (Cat. No.: AB-PK695)
Scientific Background: MARK2 is a protein-serine/threonine kinase of the CAMK group and CAMKL family. It is activated by phosphorylation on T208 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. It mediates cell polarity and microtubule function. Some substrate proteins that MARK2 phosphorylates include CRTC2/TORC2, HDAC7, DCX, KIF13B, MAPT/TAU, MAP2, MAP4, and RAB11FIP2. Phosphorylation of microtubule associated proteins leads to protein dissociation and microtubule degeneration. The phosphorylation of MARK2 by the LKB1/LKB1 complex can occur with a T208A mutation in MARK2. The ability of MARK2 to bind YWHAZ and to dissociate from the membrane occurs with a T596A mutation. Inhibition of MARK2 via small inhibitory RNA induced axon-like outgrowth from neurites.
© Kinexus Bioinformatics Corporation 2017