Product Name: ITK (509-515) pY512
Product Number: PE-04ANB90
| Size: | 200 µg | Price: | 41.00 | |
| 1 mg | $US | 82.00 | ||
| 5 mg | 194.00 |
Peptide Name: ITK (509-515) pY512
Product Use: Services as a blocking peptide for use with the ITK-pY512 rabbit polyclonal antibody (Cat. No.: AB-PK666) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. Y512 phosphorylation stimulates phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: DDQ-pY-TSS
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Product Use: Services as a blocking peptide for use with the ITK-pY512 rabbit polyclonal antibody (Cat. No.: AB-PK666) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. Y512 phosphorylation stimulates phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: DDQ-pY-TSS
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 1067.94 Da
Peptide Purity Percent after Synthesis and Purification: 90
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: ITK - pY512 phosphosite-specific antibody (Cat. No.: AB-PK666)
Peptide Molecular Mass Calculated: 1067.94 Da
Peptide Purity Percent after Synthesis and Purification: 90
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: ITK - pY512 phosphosite-specific antibody (Cat. No.: AB-PK666)
Scientific Background: ITK is a protein-serine/threonine kinase that is a member of the TK group of protein kinases in the Tec family. This kinase is widely distributed from moderate to high levels in most human tissues with highest expression in bladder, lung, lymph node, spleen, stomach, thymus, tonsils and uterus. Ligation of CD2, TCR or CD28 induces activation and tyrosine phosphorylation of ITK at Y512. ITK is expressed in T-cells and is important for T-cell development and activation through the antigen receptor. ITK require prior activation of Lck, ZAP70 and PI3-kinase for efficient activation. ITK knockout mice show multiple effects on T cell development, cytokine production and T-helper cell differentiation. T cells that lack or express mutant versions of ITK show impaired TCR-induced actin polymerization, cell polarization and regulation of the signalling events involved in cytoskeletal reorganization. ITK has been linked with the development of gastric adenocarcinomas, melanoma (metastatic) and lymphoproliferative syndrome ebv-associated autosomal type 1 (LPSA1).
© Kinexus Bioinformatics Corporation 2017