Product Name: IRAK1 (384-390) pT387
Product Number: PE-04AMY95
| Size: | 200 µg | Price: | 42.00 | |
| 1 mg | $US | 84.00 | ||
| 5 mg | 185.00 |
Peptide Name: IRAK1 (384-390) pT387
Product Use: Services as a blocking peptide for use with the IRAK1-pT387 rabbit polyclonal antibody (Cat. No.: AB-PK664) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. T387 phosphorylation stimulates phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: VRG-pT-LAY
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Product Use: Services as a blocking peptide for use with the IRAK1-pT387 rabbit polyclonal antibody (Cat. No.: AB-PK664) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. T387 phosphorylation stimulates phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: VRG-pT-LAY
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 1032.1 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: IRAK1 - pT387 phosphosite-specific antibody (Cat. No.: AB-PK664)
Peptide Molecular Mass Calculated: 1032.1 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: IRAK1 - pT387 phosphosite-specific antibody (Cat. No.: AB-PK664)
Scientific Background: IRAK1 is a protein-serine/threonine kinase of the TKL group and IRAK family. It plays a critical role in initiating innate immune response against foreign pathogens. It is involved in Toll-like receptor (TLR) and IL-1R signalling pathways, and Is rapidly recruited by MYD88 to the receptor-signalling complex upon TLR activation. Its association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. It phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. IRAK1 phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. It phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, to stimulate transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, IRAK1 translocates to the nucleus and phosphorylates STAT3. This kinase is moderate to highly expressed in most tested human tissues.
© Kinexus Bioinformatics Corporation 2017