Product Name: ASK1 (1030-1036) pS1033
Product Number: PE-04ALQ95
| Size: | 200 µg | Price: | 42.00 | |
| 1 mg | $US | 84.00 |
Peptide Name: ASK1 (1030-1036) pS1033
Product Use: Services as a blocking peptide for use with the ASK1-pS1033 rabbit polyclonal antibody (Cat. No.: AB-PK524) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located after the kinase catalytic domain. This is the major in vivo phosphorylation site in ASK1. Phosphorylation inhibits the enzymatic activity of ASK1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: APP-pS-PEE
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Product Use: Services as a blocking peptide for use with the ASK1-pS1033 rabbit polyclonal antibody (Cat. No.: AB-PK524) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located after the kinase catalytic domain. This is the major in vivo phosphorylation site in ASK1. Phosphorylation inhibits the enzymatic activity of ASK1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: APP-pS-PEE
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 979.94 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: ASK1 - pS1033 phosphosite-specific antibody (Cat. No.: AB-PK524)
Peptide Molecular Mass Calculated: 979.94 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: ASK1 - pS1033 phosphosite-specific antibody (Cat. No.: AB-PK524)
Scientific Background: ASK1 (MAP3K5, MAPKKK5) is a protein-serine/threonine kinase that is a member of the STE group of protein kinases in the STE11 family. This kinase is moderate to highly expressed in most tested human tissues. ASK1 regulates apoptosis through mitochondria-dependent caspase activation. Overexpression of ASK1 induces apoptotic cell death. ASK1 interacts with members of the TRAF family and is activated by TRAF2 in the tumour necrosis factor-alpha (TNF) signalling pathway. ASK1 is activated by phosphorylation at T838 by MAP3K6 and inhibited by phosphorylation at S966 and S1033. It acts as an upstream activator of the MKK>JNK and MKK>p38 MAPK signal transduction cascades through the direct phosphorylation of different MAP kinases, such as MAP2K4 (MKK4), MAP2K3 (MKK3), MAP2K6 (MKK6), and MAP2K7 (MKK7). It is dephosphorylated and activated by PGAM5. ASK1 contains an N-terminal autoinhibitory domain. It is inhibited by phosphorylation at S966 and S1033, and by HIV-1 Nef. ASK1 appears to be a tumour suppressor protein (TSP) as the active form of the protein kinase normally acts to inhibit tumour cell proliferation. 24% of melanoma cell lines were found to contain mutations in the coding-regions of either MAP3K5 or the closely related kinase MAP3K9, often in the kinase catalytic domain.Loss of heterozygosity in MAP3K5 was observed in 85% of melanoma specimens, indicating that the previously observed mutations result in the loss-of-function in the protein. An I780F substitution, the most commonly observed mutant form of the kinase, inhibits phosphotransferase activity.
© Kinexus Bioinformatics Corporation 2017