Product Name: Chk1 (342-348) pS345
Product Number: PE-04AJU95
| Size: | 200 µg | Price: | 37.00 | |
| 1 mg | $US | 74.00 | ||
| 5 mg | 174.00 |
Peptide Name: Chk1 (342-348) pS345
Product Use: Services as a blocking peptide for use with the Chk1-pS345 rabbit polyclonal antibody (Cat. No.: AB-PK579) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the C-terminal half of the kinase after the catalytic domain. S345 phosphorylation stimulates phosphotransferase activity. Phosphorylation regulates apoptosis, cell cycle progression, intracellular location, and protein degradation. It induces binding to 14-3-3-sigma, claspin, p21Cip1 and p53.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: ISF-pS-QPT
Product Use: Services as a blocking peptide for use with the Chk1-pS345 rabbit polyclonal antibody (Cat. No.: AB-PK579) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the C-terminal half of the kinase after the catalytic domain. S345 phosphorylation stimulates phosphotransferase activity. Phosphorylation regulates apoptosis, cell cycle progression, intracellular location, and protein degradation. It induces binding to 14-3-3-sigma, claspin, p21Cip1 and p53.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: ISF-pS-QPT
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 1033.05 Da
Peptide Purity Percent after Synthesis and Purification: >90
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: Chk1 - pS345 phosphosite-specific antibody (Cat. No.: AB-PK579)
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 1033.05 Da
Peptide Purity Percent after Synthesis and Purification: >90
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: Chk1 - pS345 phosphosite-specific antibody (Cat. No.: AB-PK579)
Scientific Background: Chk1 (CHEK1) is a protein-serine/threonine kinase of the CAMK group and CAMKL family. It is required for checkpoint-mediated cell cycle arrest. It appears to be a tumour suppressor protein (TSP). The active form of the protein kinase normally acts to inhibit tumour cell proliferation. It activates DNA repair (i.e. homologous recombination) by phosphorylating RAD51 or FANCE in response to the presence of damage or unreplicated DNA. It regulates cell cycle progression via several mechanisms: 1) phosphorylation of certain proteins such as CDC25A, CDC25B and CDC25C, promoting their degradation; 2) phosphorylation and activation of p53; 3) phosphorylation and inactivation of TLK1, which consequently prevents TLK1-dependent phosphorylation of chromatin assembly factor ASF1A; 4) phosphorylation of histones and regulating transcription of genes involved in cell-cycle progression regulation; 5) phosphorylation of RB1 to promote its interaction with E2F transcription factors and subsequent cell cycle arrest. It is nhibited by phosphorylation at S280. S345A mutation of Chk1 leads to reduced phosphorylation and impaired activation to nuclear retention after checkpoint activation. Mutations at 372, 376, and 379 amino acids lead to misfolding and induced binding with ubiquitin.
© Kinexus Bioinformatics Corporation 2017