Product Name: BRSK1 (186-192) pT189
Product Number: PE-04AIW95
| Size: | 200 µg | Price: | 42.00 | |
| 1 mg | $US | 84.00 |
Peptide Name: BRSK1 (186-192) pT189
Product Use: Services as a blocking peptide for use with the BRSK1-pT189 rabbit polyclonal antibody (Cat. No.: AB-PK549) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. T189 phosphorylation stimulates phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: LLE-pT-SCG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Product Use: Services as a blocking peptide for use with the BRSK1-pT189 rabbit polyclonal antibody (Cat. No.: AB-PK549) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. T189 phosphorylation stimulates phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: LLE-pT-SCG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 975.02 Da
Peptide Purity Percent after Synthesis and Purification: 95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: BRSK1 - pT189 phosphosite-specific antibody (Cat. No.: AB-PK549)
Peptide Molecular Mass Calculated: 975.02 Da
Peptide Purity Percent after Synthesis and Purification: 95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: BRSK1 - pT189 phosphosite-specific antibody (Cat. No.: AB-PK549)
Scientific Background: BRSK1 is a protein-serine/threonine kinase of the CAMK group and CAMKL family. It is activated by phosphorylation on T205 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Its substrates include CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Its phosphotransferase activity is lost with the K59A, and G327A mutations. It is involved in the UV-induced DNA damage checkpoint response, likely by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C. A LKB1/LKB1 complex with the STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39 phosphorylates and activates BRSK1, but this can be prevented by an inhibitory T189A mutation.
© Kinexus Bioinformatics Corporation 2017