Product Name: A-Raf (299-305) pY302
Product Number: PE-04AFW99
| Size: | 200 µg | Price: | 47.00 | |
| 1 mg | $US | 94.00 | ||
| 5 mg | 206.00 |
Peptide Name: A-Raf (299-305) pY302
Product Use: Services as a blocking peptide for use with the A-Raf-pY302 rabbit polyclonal antibody (Cat. No.: AB-PK500) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located just before the kinase catalytic domain. Y302 phosphorylation stimulates phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: SGY-pY-WEV
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Product Use: Services as a blocking peptide for use with the A-Raf-pY302 rabbit polyclonal antibody (Cat. No.: AB-PK500) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located just before the kinase catalytic domain. Y302 phosphorylation stimulates phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: SGY-pY-WEV
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 1157.12 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: A-Raf - pY302 phosphosite-specific antibody (Cat. No.: AB-PK500)
Peptide Molecular Mass Calculated: 1157.12 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: A-Raf - pY302 phosphosite-specific antibody (Cat. No.: AB-PK500)
Scientific Background: A-Raf (RafA) is a protein-serine/threonine kinase of the TKL group and RAF family. It is involved in the transduction of mitogenic signals from the cell membrane receptors via Ras and MEK1/2 to the nucleus. It has been implicated in playing a role in the postsynaptic responses of hippocampal neuron. Phosphorylation of S214, S257, S262, S264, Y301, Y302, S432, T442, T452, T455 and S582 increases phosphotransferase activity. It is inhibited by Nexavar/Sorafenib (IC50>50 nM). A-Raf may be an oncoprotein (OP) based on its similarity to Raf1 and B-Raf. However, it does not display a higher than normal rate of mutation than most proteins in human tumours. The active form of the protein kinase acts to promote tumour cell proliferation.
© Kinexus Bioinformatics Corporation 2017