Product Name: PKCd (504-510) pT507
Product Number: PE-04ADZ99
| Size: | 200 µg | Price: | 42.00 | |
| 1 mg | $US | 84.00 | ||
| 5 mg | 185.00 |
Peptide Name: PKCd (504-510) pT507
Product Use: Services as a blocking peptide for use with the PKCd-pT507 rabbit polyclonal antibody (Cat. No.: AB-PK767) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. T507 phosphorylation stimulates phosphotransferase activity and degradation.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: RAS-pT-FCG
Peptide Modifications N Terminus: Free amino
Product Use: Services as a blocking peptide for use with the PKCd-pT507 rabbit polyclonal antibody (Cat. No.: AB-PK767) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. T507 phosphorylation stimulates phosphotransferase activity and degradation.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: RAS-pT-FCG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 994.04 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: PKCd - pT507 phosphosite-specific antibody (Cat. No.: AB-PK767)
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 994.04 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: PKCd - pT507 phosphosite-specific antibody (Cat. No.: AB-PK767)
Scientific Background: PKCd (PRKCD, PKC-delta) is a protein-serine/threonine kinase in the novel protein kinase C family. It is dependent on acidic phospholipids (e.g. phosphatidylserine) and diacylglycerol for full phosphotransferase activity, and does not require calcium. It is highly expressed and widely distributed in most tested human tissues, and is a major isoform of PKC expressed in hemopoietic cells. It is involved in promoting apoptosis, differentiation of cell types, cell growth, regulation of transcription factors, NFkB signalling induced by reactive oxygen species, and antigen presentation. PKCd is activated by phosphorylation at Y313, Y514, Y525, and S645. Phosphorylation of T507 increases phosphotransferase activity and induces degradation of PKC-delta. Phosphorylation of Y52, Y64, Y187, and Y567 inhibits phosphotransferase activity. Phosphatidylinositol is the most potent activator of PKCd. PKC delta is involved in fundamental cellular functions regulated by diacylglycerols, which is mimicked by phorbol esters. PKCd may be a tumour suppressor protein (TSP). PKCd negatively regulates cell proliferation in colon cell lines. It is also required for ErbB2-driven mammary gland tumourigenesis. Overexpression is seen in breast cancer and is associated with tamoxifen resistance. Reduced expression in MCF-7 cells is associated with a metastatic phenotype.
© Kinexus Bioinformatics Corporation 2017