Product Name: MAPKAPK5 (183-190) pT186
Product Number: PE-04AAS95
| Size: | 200 µg | Price: | 45.00 | |
| 1 mg | $US | 91.00 | ||
| 5 mg | 199.00 |
Peptide Name: MAPKAPK5 (183-190) pT186
Product Use: Services as a blocking peptide for use with the MAPKAPK5-pT186 rabbit polyclonal antibody (Cat. No.: AB-PK693) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. T186 phosphorylation is predicted to be stimulatory for phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: PQF-pT-PYYV
Peptide Modifications N Terminus: Free amino
Product Use: Services as a blocking peptide for use with the MAPKAPK5-pT186 rabbit polyclonal antibody (Cat. No.: AB-PK693) that is also available from Kinexus. This phosphopeptide may also be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the protein kinase catalytic domain activation T loop region between subdomains VII and VIII. T186 phosphorylation is predicted to be stimulatory for phosphotransferase activity.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: PQF-pT-PYYV
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: βAla-Cys
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 1307.4 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: MAPKAPK5 - pT186 phosphosite-specific antibody (Cat. No.: AB-PK693)
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 1307.4 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Related Product 1: MAPKAPK5 - pT186 phosphosite-specific antibody (Cat. No.: AB-PK693)
Scientific Background: MAPKAPK5 is a protein-serine/threonine kinase of the CAMK group and MAPKAPK family. p38 alpha and beta-dependent phosphorylation increases its activity. It is activated by stress-related extracellular stimuli; such as H2O2, arsenite, anisomycin TNF alpha and also PMA and the calcium ionophore A23187; but to a lesser extent. In vitro, activated by SQSTM1. T182A substitution leads to no p38-beta/MAPK11-induced activation. In addition, T182D, S212D and L337G mutations are associated with higher protein kinase activity. It appears to be a tumour suppressor protein (TSP) that mediates Ras-induced senescence and phosphorylates p53/TP53. It also phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, and RHEB.
© Kinexus Bioinformatics Corporation 2017