Product Name: FGG(434-453) pY444+pY448
Product Number: PE-04AAL95
Size: 200 µg      Price:99.00
1 mg      $US198.00
5 mg      434.00
Peptide Name: FGG(434-453) pY444+pY448

Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases. The peptide sequence is located in the C-terminus.

Peptide Production Method: Solid-phase peptide synthesis

Peptide Origin: Homo sapiens

Peptide Sequence: VRPEHPAETE-pY-DSL-pY-PEDDL

Peptide Modifications N Terminus: Acetyl

Peptide Modifications C Terminus: Amide

Peptide Modifications Other: Phosphorylated

Peptide Molecular Mass Calculated: 2576.4 Da

Peptide Purity Percent after Synthesis and Purification: >95

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Scientific Background: This peptide is amino acids 408 to 427 fragment of Fibrinogen ?? chain, the elongated C-terminal portion resulting from the alternative splicing of fibrinogen ? chain mRNA that retains the last intron. This inserted sequence reduces thrombin-induced platelet aggregation by binding to the thrombin anion-binding exosite (ABE)-II and interrupting interactions with platelet receptors, including glycoprotein Ib? (GpIb?) and protease-activated receptor (PAR) 1. The presence of the ?? chain variant also appears to alter clot structure.
References

[1] Lancellotti S, Rutella S, Filippis VD, Pozzi N, Rocca B & Cristofaro RD (2008). Fibrinogen-elongated γ Chain Inhibits Thrombin-induced Platelet Response, Hindering the Interaction with Different Receptors. J Biol Chem 283(44), 30193-204.PMC2662079.

[2] Abdel Azi, M.H., Sidhu, P.S., Liang, Aiye, Kim, J.Y., Mosier, P.D., Zhou, Q., Farrell, D.H., Desai, U.R. Designing allosteric regulators of thrombin. Monosulfated benzofuran dimers selectively interact with Arg173 of exosite II to induce inhibition. J Med Chem. 2012 August 9; 55(15): 6888–6897. doi:10.1021/jm300670q. PMCID: PMC3416887

[3] Gersh, K.C., Nagaswami, C., Weisel, J.W., Lord, S.T. The presence of γ’ chain impairs fibrin polymerization. Thromb Res. 2009 July; 124(3): 356–363. doi:10.1016/j.thromres.2008.11.016. PMCID: PMC2752440.