Product Name: Akt-sub KinSub, biotinyl.
Product Number: PE-03AAG95
Size: 200 µg      Price:40.00
1 mg      $US79.00
Peptide Name: Akt-sub KinSub, biotinyl.

Product Use: For assaying the phosphotransferase activity of RAC-alpha serine/threonine-protein kinase (Akt1, UniProt ID P31749).

Peptide Production Method: Solid-phase peptide synthesis

Peptide Sequence: KRPRAASFAE

Peptide Modifications N Terminus: Biotin-βAla

Peptide Modifications C Terminus: Amide

Peptide Molecular Mass Calculated: 1485.7 Da

Peptide Purity Percent after Synthesis and Purification: >95

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Peptide Recommended Enzyme: Akt isoforms

Scientific Background: Akt1 (PKBA) is a protein-serine/threonine kinase of the AGC group and Akt family. It is normally inactive in the cytoplasm. When PI3K is activated by extracellular growth factors, Akt1 is recruited to the cell membrane and phosphorylated by PDK1, mTOR and other protein kinases to become active. This leads to the induction of survival, proliferation, angiogenesis and metabolism pathways. Akt1 is also involved in cell-cycle progression via its inhibitory phosphorylation of cyclin-dependent kinases and activatory phosphorylation of mTOR. This kinase is highly expressed in most tested human tissues. Orthologues of Akt are conserved in a wide range of animals, plants and unicellular eukaryotes. Phosphorylation of T72, S124,S129, S246, T308, Y315,Y326, T450, S473 and Y474 increases Akt1 phosphotransferase activity. TRAF6-induced 'K63'-linked Akt1 ubiquitination is critical for phosphorylation and activation. Phosphorylation of T34 inhibits phosphotransferase activity. Akt1 has been implicated in a wide range of human cancers, including lymphomas, breast, lung, colorectal, pancreatic and prostate cancers.