Product Name: CREB1 (123-135) KinSub, biotinyl.
Product Number: PE-03AAF90
Size: 200 µg      Price:43.00
1 mg      $US86.00
5 mg      203.00
Peptide Name: CREB1 (123-135) KinSub, biotinyl.

Product Use: For assaying the phosphotransferase activity of CAMK1b, CAMK1d, CAMK1g, CAMK4 and PKCa.

Peptide Production Method: Solid-phase peptide synthesis

Peptide Sequence: KRREILSRRPSYR

Peptide Modifications N Terminus: Biotin-βAla

Peptide Modifications C Terminus: Amide

Peptide Molecular Mass Calculated: 2070.4 Da

Peptide Purity Percent after Synthesis and Purification: >90

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Peptide Recommended Enzyme: PNCK; CAMK1b; CAMK1d; CAMK1g; CAMK4; PKA (PRKA); PKCa

Scientific Background: PKACb is one of several protein kinases that can phosphorylate CREBtide. Human PKACb (also known as PRKACB) is a protein-serine/threonine kinase of 351 amino acid length, with a predicted molecular mass of 40,623 Da. It is a member of the AGC group of protein kinases in the PKA family. This kinase is highly expressed and widely distributed in most tested human tissues. Orthologues of PKACb are amongst the most highly conserved protein kinases in animals, plants, fungi and unicellular eukaryotes. PKACb is activated by binding of two cAMP molecules to each of the two associated regulatory subunits in the PKA holoenzyme. Binding of cAMP induces dissociation of the two active catalytic subunits. Phosphorylation of T198 increases phosphotransferase activity. Phosphorylation of S339 plays a key role in stabilizing PKA. PKACb has been assigned to human chromosome region 1p36.1 (1). At the amino acid level C-alpha and C-beta showed 93% homology.
References

[1] PMID: 12574406; Bok J, Zha XM, Cho YS, Green SH.; An extranuclear locus of cAMP-dependent protein kinase action is necessary and sufficient for promotion of spiral ganglion neuronal survival by cAMP.; J Neurosci. 2003 Feb 1;23(3):777-87.