Product Name: IRAKSelectide
Product Number: PE-01BHQ90
Size: 200 µg      Price:48.00
1 mg      $US96.00
5 mg      227.00
Peptide Name: IRAKSelectide

Product Use: For assaying the phosphotransferase activity of Interleukin 1 receptor-associated kinase 1 (Pelle-like protein kinase) (UniProt ID P51617).

Peptide Production Method: Solid-phase peptide synthesis

Peptide Sequence: KKQQQKFITMVVIVA

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: βAla-Cys

Peptide Molecular Mass Calculated: 1935.39 Da

Peptide Purity Percent after Synthesis and Purification: >90

Peptide Appearance: White powder

Peptide Form: Solid
Storage Conditions: -20°C

Peptide Recommended Enzyme: IRAK

Scientific Background: IRAK1 is a protein-serine/threonine kinase of the TKL group and IRAK family. It plays a critical role in initiating innate immune response against foreign pathogens. It is involved in Toll-like receptor (TLR) and IL-1R signalling pathways, and Is rapidly recruited by MYD88 to the receptor-signalling complex upon TLR activation. Its association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. It phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. IRAK1 phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. It phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, to stimulate transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, IRAK1 translocates to the nucleus and phosphorylates STAT3. This kinase is moderate to highly expressed in most tested human tissues.