Product Name: AMPKSelectide
Product Number: PE-01BGH95
Size: 200 µg      Price:45.00
1 mg      $US91.00
5 mg      214.00
Peptide Name: AMPKSelectide

Product Use: For assaying the phosphotransferase activity of 5'-AMP-activated protein kinase catalytic subunit alpha-1 (UniProt ID Q13131).

Peptide Production Method: Solid-phase peptide synthesis

Peptide Sequence: KKLPLTRSHNNFVA

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: βAla-Cys

Peptide Molecular Mass Calculated: 1799.2 Da

Peptide Purity Percent after Synthesis and Purification: >90

Peptide Appearance: White powder

Peptide Form: Solid
Storage Conditions: -20°C

Peptide Recommended Enzyme: AMPK

Scientific Background: AMPKa1 (PRKAA1) is a protein-serine/threonine kinase of the CAMK group and CAMKL family. AMP-activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis. AMPK exists as a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits. Binding of AMP results in allosteric activation, inducing phosphorylation on T183 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. AMPK is activated in response to stresses that deplete cellular ATP (low glucose, hypoxia and ischemia) and via signalling pathways in response to adiponectin, leptin and CAMKK-beta. Phosphorylation at T174 by CAMKK1 and CAMKK2 increases phosphotransferase activity, triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio.