Product Name: ATM (3045-3059)
Product Number: PE-01ARH95
Size: 200 µg      Price:51.00
1 mg      $US102.00
5 mg      224.00
Peptide Name: ATM (3045-3059)

Product Use: Services as a blocking peptide for use with the ATM-3 rabbit polyclonal antibody (Cat. No.: AB-NK230-3) that is also available from Kinexus. The peptide sequence is located in the C-terminus.

Peptide Production Method: Solid-phase peptide synthesis

Peptide Origin: Homo sapiens

Peptide Sequence: CLIQQAIDPKNLSRLF

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: Free carboxyl

Peptide Molecular Mass Calculated: 1858.2 Da

Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Related Product 1: ATM pan-specific antibody (Cat. No.: AB-NK230-3)

Scientific Background: ATM is a protein-serine/threonine kinase of the Atypical group and PIKK family. Phosphorylation at S1981 increases phosphotransferase activity and induces interaction with ATM, NBS1 and p53. It acts as a DNA damage sensor and appears to be a tumour suppressor protein (TSP). Cancer-related mutations in human tumours point to a loss of function of the protein kinase. The active form of the protein kinase normally acts to inhibit tumour cell proliferation. It regulates cell cycle progression checkpoints and induces DNA repair. It activates checkpoint signalling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA). It recognizes the substrate consensus sequence [S/T]-Q in many substrates. It phosphorylates and activates p53, while also inactivating the p53-inhibitor: MDM2. It phosphorylates S139 of the histone variant H2AX/H2AFX at double strand breaks (DSBs), which regulates the DNA damage response mechanism.. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, ATM acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, and prevents accessibility to the RAG complex and recombination of the second allele. It binds DNA ends, and it is part of the BRCA1- associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex. This association appears to be a dynamic process changing throughout the cell cycle and within subnuclear domains. DNA damage promotes association with RAD17. It also phosphorylates p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. It is involved in the activation of ABL1 and JNK. It may function in vesicle and/or protein transport, replication-dependent histone mRNA degradation as well as have roles in T-cell development, gonad and neurological function.