Product Name: Alk (1272-1278)
Product Number: PE-01AQQ01
Size: 200 µg      Price:26.00
1 mg      $US52.00
5 mg      121.00
Peptide Name: Alk (1272-1278)

Product Use: The peptide sequence is located in the protein kinase catalytic domain activation T-loop between subdomains VII and VIII. The R1275Q mutation is common in human cancers.

Peptide Production Method: Solid-phase peptide synthesis

Peptide Origin: Homo sapiens

Peptide Sequence: GMAQDIY

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: βAla-Cys

Peptide Molecular Mass Calculated: 970.1 Da

Peptide Purity Percent after Synthesis and Purification: >90

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Storage Stability: stable

Scientific Background: Alk is a protein-tyrosine kinase of the TK group and Alk family. It is a neuronal receptor for the ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor. Alk signals via MyD88 and is important in neuronal differentiation and development. Cancer-related mutations in human tumours point to a gain of function of the protein kinase. The active form of the protein kinase normally acts to promote tumour cell proliferation. Chromosomal rearrangments (most common), mutations, and amplifications with the Alk gene are associated with numerous tumours including: anaplastic large cell lymphomas, neuroblastomas, and non-small cell lung cancer. These fusion proteins, as well as other mutations and amplifications, likely confer heightened (possibly consititutive) phosphotransferase activity and promote cell growth and anti-apoptotic pathways such as the Akt and MAPK pathways. In Neuroblastoma 3 (NBLST3), there was constitutive activation, and Endoplasmic Reticulum or Golgi Apparatus localization when Alk had gain of function mutations with F1174I or F1174V (located in the kinase catalytic subdomain III) or R1275Q (located just before the kinase catalytic Subdomain VII). Translocations of the Alk gene resulting in fusion proteins with NPM1 are observed in 5-10% of non-Hodgkin lymphomas, with CARS and SEC31A in inflammatory myofibroblastic tumours (IMTs) and ALO17 in anaplastic large-cell lymphoma (ALCL). The genes C/EBPB, and BCL2A1 are essential for ALCL growth and transcriptionally induced by Alk.