Product Name: KinSubaRRKSF
Product Number: PE-01ALS95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSubaRRKSF
Product Use: For assaying the phosphotransferase activity of Homeodomain-interacting protein kinase 4 (HIPK4, UniProt ID Q8NE63). The KinSubaRRKSF peptide demonstrated very high phosphotransferase activity with CHK2, and exhibited very low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSubaRRKSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRGRRKSFRRIGHG
Product Use: For assaying the phosphotransferase activity of Homeodomain-interacting protein kinase 4 (HIPK4, UniProt ID Q8NE63). The KinSubaRRKSF peptide demonstrated very high phosphotransferase activity with CHK2, and exhibited very low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSubaRRKSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRGRRKSFRRIGHG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1696 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: CHK2
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1696 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: CHK2
Scientific Background: HIPK4 is one of several protein kinases that can phosphorylate KinSubaRRKSF. Human HIPK4 is a protein-serine/threonine kinase of 616 amino acid length, with a predicted molecular mass of 69,425 Da. It is a member of the CMGC group of protein kinases in the DYRK family, and HIPK subfamily. Orthologues of HIPK4 are highly conserved in mammals. HIPK4 can act as a co-repressor of transcription factors (1). HIPK4 has been shown to phosphorylate the human p53 protein at S9, both in vitro and in vivo which is important for p53 mediated transcriptional repression. RT-PCR analysis showed moderate expression of HIPK4 in lung and white adipose tissue and weak expression in brain and liver. HIPK4 expression has also been observed in various cancer cell lines (2).
References
[1] Arai S, Matsushita A, Du K, Yagi K, Okazaki Y, Kurokawa R. Novel homeodomain-interacting protein kinase family member, HIPK4, phosphorylates human p53 at serine 9. FEBS Lett. 2007 Dec 11;581(29):5649-57. PMID: 18022393.
[2] Di Stefano V, Rinaldo C, Sacchi A, Soddu S, D'Orazi G. Homeodomain-interacting protein kinase-2 activity and p53 phosphorylation are critical events for cisplatin-mediated apoptosis. Exp Cell Res. 2004 Feb 15;293(2):311-20. PMID: 14729469.
[1] Arai S, Matsushita A, Du K, Yagi K, Okazaki Y, Kurokawa R. Novel homeodomain-interacting protein kinase family member, HIPK4, phosphorylates human p53 at serine 9. FEBS Lett. 2007 Dec 11;581(29):5649-57. PMID: 18022393.
[2] Di Stefano V, Rinaldo C, Sacchi A, Soddu S, D'Orazi G. Homeodomain-interacting protein kinase-2 activity and p53 phosphorylation are critical events for cisplatin-mediated apoptosis. Exp Cell Res. 2004 Feb 15;293(2):311-20. PMID: 14729469.
© Kinexus Bioinformatics Corporation 2017