Product Name: KinSub8RRGSF
Product Number: PE-01ALM95
Size: 200 µg      Price:99.00
      $US
Peptide Name: KinSub8RRGSF

Product Use: For assaying the phosphotransferase activity of Serine/threonine-protein kinase Sgk3 (UniProt ID Q96BR1). The KinSub8RRGSF peptide demonstrated high phosphotransferase activity with SGK3, and exhibited low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.

Peptide Production Method: Solid-phase peptide synthesis

Peptide Origin: KinSub8RRGSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.

Peptide Sequence: GFLSRRGSFGNGKHG

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: Amide

Peptide Molecular Mass Calculated: 1575.8 Da

Peptide Purity Percent after Synthesis and Purification: >95

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Peptide Recommended Enzyme: SGK3

Scientific Background: SGK3 is one of several protein kinases that can phosphorylate KinSub8RRGSF. Human SGK3 is a protein-serine/threonine kinase of 496 amino acid length, with a predicted molecular mass of 57,108 Da. It is a member of the AGC group of protein kinases in the SGK family. This kinase is highly expressed and widely distributed in most tested human tissues. Orthologues of SGK3 are highly conserved in vertebrates, including amphibians. SGK3 features a PX (phox homology) domain. SGK3 is activated through phosphorylation of T320 and S486. PKD1 can phosphorylate and activate SGK3 in vitro (1). A 10-fold increase in PKD1 increases the phosphorylation status of SGK3. When expressed in human embryonic kidney cells, IGF1 and peroxide significantly activate SGK3, and the activation could be reduced by preincubation with inhibitors of PI3 kinase. A yeast 2-hybrid screen found direct interaction between human SGK3 and GSK-beta. SGK3 phosphorylates several target proteins and has a role in neutral amino acid transport and activation of potassium and chloride channels (2). SGK3 has been linked with the development of breast carcinomas.
References

[1] Kobayashi T, Deak M, Morrice N, Cohen P. Characterization of the structure and regulation of two novel isoforms of serum- and glucocorticoid-induced protein kinase. Biochem J. 1999 Nov 15;344 Pt 1:189-97. PMID:10548550 PMCID: PMC1220630

[2] Gamper N, Fillon S, Feng Y, Friedrich B, Lang PA, Henke G, Huber SM, Kobayashi T, Cohen P, Lang F. K+ channel activation by all three isoforms of serum- and glucocorticoid-dependent protein kinase SGK. Pflugers Arch. 2002 Oct;445(1):60-6. Epub 2002 Aug 28. PMID: 12397388