Product Name: KinSub6RRLSP
Product Number: PE-01ALH95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub6RRLSP
Product Use: For assaying the phosphotransferase activity of calcium/calmodulin-dependent protein-serine kinase 2 gamma (CAMK2g, UniProt ID Q13555). The KinSub6RRLSP peptide demonstrated high phosphotransferase activity with CAMK2g, and exhibited moderate specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub6RRLSP was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: HIRGRRLSPGVVGIR
Product Use: For assaying the phosphotransferase activity of calcium/calmodulin-dependent protein-serine kinase 2 gamma (CAMK2g, UniProt ID Q13555). The KinSub6RRLSP peptide demonstrated high phosphotransferase activity with CAMK2g, and exhibited moderate specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub6RRLSP was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: HIRGRRLSPGVVGIR
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1672 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: CAMK2g
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1672 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: CAMK2g
Scientific Background: CAMK2g is one of several protein kinases that can phosphorylate KinSub6RRLSP. Human CaMK2g is a protein-serine/threonine kinase of 558 amino acid length, with a predicted molecular mass of 62,609 Da. It is a member of the CAMK group of protein kinases in the CAMK2 family. This kinase is highly expressed and widely distributed in most tested human tissues. Orthologues of CaMK2g are highly conserved in animals and plants. CAMK2 is composed of up to four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. CaMK2g is activated by Ca2+/calmodulin to autophosphorylate at T287, which then stimulates its phosphotransferase activity in a Ca2+-independent manner. CAMK2g has six alternatively spliced variants that encode six different isoforms. Some of these variants have been identified in human tumours (1). Transgenic mice expressing a partially calcium-independent mutant form of CAMK2g showed 1.5- to 2-fold increase in the thymus of these mice, at least in part due to an increase in the life span of double-positive thymocytes (2). There was an increase in the number of T cells in the secondary lymphoid organs that had acquired an antigen-dependent memory phenotype.
References
[1] Tombes RM, Krystal GW. Identification of novel human tumor cell-specific CaMK-II variants. Biochim Biophys Acta. 1997 Mar 1;1355(3):281-92. PMID: 9060999.
[2] Bui JD, Calbo S, Hayden-Martinez K, Kane LP, Gardner P, Hedrick SM. A role for CaMKII in T cell memory. Cell. 2000 Feb 18;100(4):457-67. PMID: 10693762.
[1] Tombes RM, Krystal GW. Identification of novel human tumor cell-specific CaMK-II variants. Biochim Biophys Acta. 1997 Mar 1;1355(3):281-92. PMID: 9060999.
[2] Bui JD, Calbo S, Hayden-Martinez K, Kane LP, Gardner P, Hedrick SM. A role for CaMKII in T cell memory. Cell. 2000 Feb 18;100(4):457-67. PMID: 10693762.
© Kinexus Bioinformatics Corporation 2017