Product Name: KinSub6RRGSF
Product Number: PE-01ALF95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub6RRGSF
Product Use: For assaying the phosphotransferase activity of Thousand and one amino acid protein 2 (TAO2, UniProt ID Q9UL54). The KinSub6RRGSF peptide demonstrated high phosphotransferase activity with PAK5 (PAK7), and exhibited high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub6RRGSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRGRRGSFGGGGHG
Product Use: For assaying the phosphotransferase activity of Thousand and one amino acid protein 2 (TAO2, UniProt ID Q9UL54). The KinSub6RRGSF peptide demonstrated high phosphotransferase activity with PAK5 (PAK7), and exhibited high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub6RRGSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRGRRGSFGGGGHG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1370.5 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: PAK5 (PAK7)
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1370.5 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: PAK5 (PAK7)
Scientific Background: TAO2 is one of several protein kinases that can phosphorylate KinSub6RRGSF. Human TAO2 (also known as TAOK2) is a protein-serine/threonine kinase of 1235 amino acid length, with a predicted molecular mass of 138,251 Da. It is a member of the STE group of protein kinases in the STE20 family, and TAO subfamily. This kinase is highly expressed and widely distributed in most tested human tissues except in the brain and spinal cord where it is poorly expressed. Orthologues of TAO2 are highly conserved in vertebrates, including amphibians. TAO2 interacts with and activates MKK3 and MKK6, which phosphorylate and activate p38 MAPK (1). It also interfers with MAP3K7-binding to IKKa and thus prevents NF-kappa-B activation. TAO2 may play an important role in the activation of specific intracellular signalling pathways that are unique to osmotic stress (2).
References
[1] Hutchison M, Berman KS, Cobb MH. Isolation of TAO1, a protein kinase that activates MEKs in stress-activated protein kinase cascades. J Biol Chem. 1998 Oct 30;273(44):28625-32. PMID: 9786855
[2] Chen Z, Raman M, Chen L, Lee SF, Gilman AG, Cobb MH. TAO (thousand-and-one amino acid) protein kinases mediate signaling from carbachol to p38 mitogen-activated protein kinase and ternary complex factors. J Biol Chem. 2003 Jun 20;278(25):22278-83. Epub 2003 Mar 28. PMID: 12665513
[1] Hutchison M, Berman KS, Cobb MH. Isolation of TAO1, a protein kinase that activates MEKs in stress-activated protein kinase cascades. J Biol Chem. 1998 Oct 30;273(44):28625-32. PMID: 9786855
[2] Chen Z, Raman M, Chen L, Lee SF, Gilman AG, Cobb MH. TAO (thousand-and-one amino acid) protein kinases mediate signaling from carbachol to p38 mitogen-activated protein kinase and ternary complex factors. J Biol Chem. 2003 Jun 20;278(25):22278-83. Epub 2003 Mar 28. PMID: 12665513
© Kinexus Bioinformatics Corporation 2017