Product Name: KinSub5RRGSF
Product Number: PE-01ALB95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub5RRGSF
Product Use: For assaying the phosphotransferase activity of Dual specificity protein kinase CLK1 (UniProt ID P49759). The KinSub5RRGSF peptide demonstrated moderate phosphotransferase activity with CLK1, and exhibited very high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub5RRGSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRSRRGSFRGGGGG
Product Use: For assaying the phosphotransferase activity of Dual specificity protein kinase CLK1 (UniProt ID P49759). The KinSub5RRGSF peptide demonstrated moderate phosphotransferase activity with CLK1, and exhibited very high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub5RRGSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRSRRGSFRGGGGG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1419.5 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: CLK1
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1419.5 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: CLK1
Scientific Background: CLK1 is one of several protein kinases that can phosphorylate KinSub5RRGSF. Human CLK1(also known as STY) is a protein-serine/threonine kinase of 484 amino acid length, with a predicted molecular mass of 57,291 Da. It is a member of the CMGC group of protein kinases in the CLK family. This kinase shows high variability in human tissue distribution with the highest levels detected in adrenals, brain, kidney, lung, ovary, pituitary, prostate, spinal cord, testes, thymus, thyroid, trachea, and uterus. Orthologues of CLK1 are amongst the most highly conserved protein kinases in animals, plants, fungi and unicellular eukaryotes. CLK1 interacts with PPIG. It is a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases. The phosphorylated serine/arginine-rich (SR) proteins involves in the pre-mRNA processing and releasing through nucleus into nucleoplasm. CLK1, which could phosphorylates the specific SR proteins (1) such as ASF/SF2, may directly regulates the activity and compartmentalization of SR splicing factors (2).
References
[1] Prasad J, Manley JL. Regulation and substrate specificity of the SR protein kinase Clk/Sty. Mol Cell Biol. 2003 Jun;23(12):4139-49. PMID: 12773558.
[2] Colwill K, Feng LL, Yeakley JM, Gish GD, Cáceres JF, Pawson T, Fu XD. SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/arginine-rich splicing factors. J Biol Chem. 1996 Oct 4;271(40):24569-75. PMID: 8798720.
[1] Prasad J, Manley JL. Regulation and substrate specificity of the SR protein kinase Clk/Sty. Mol Cell Biol. 2003 Jun;23(12):4139-49. PMID: 12773558.
[2] Colwill K, Feng LL, Yeakley JM, Gish GD, Cáceres JF, Pawson T, Fu XD. SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/arginine-rich splicing factors. J Biol Chem. 1996 Oct 4;271(40):24569-75. PMID: 8798720.
© Kinexus Bioinformatics Corporation 2017