Product Name: KinSub5RRDSP
Product Number: PE-01ALA95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub5RRDSP
Product Use: For assaying the phosphotransferase activity of Proto-oncogene serine/threonine-protein kinase Pim-1 (UniProt ID P11309). The KinSub5RRDSP peptide demonstrated high phosphotransferase activity with Pim1, and exhibited very high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub5RRDSP was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: HGRVRRDSPGGFGYG
Product Use: For assaying the phosphotransferase activity of Proto-oncogene serine/threonine-protein kinase Pim-1 (UniProt ID P11309). The KinSub5RRDSP peptide demonstrated high phosphotransferase activity with Pim1, and exhibited very high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub5RRDSP was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: HGRVRRDSPGGFGYG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1616.8 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Pim1
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1616.8 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Pim1
Scientific Background: Pim1 is one of several protein kinases that can phosphorylate KinSub5RRDSP. Human Pim1 is a protein-serine/threonine kinase of 404 amino acid length, with a predicted molecular mass of 45,412 Da. It is a member of the CAMK group of protein kinases in the Pim family. This kinase is highly expressed and widely distributed in most tested human tissues. Orthologues of Pim1 are highly conserved in vertebrates and insects. Pim1 was originally identified from Moloney murine leukemia virus induced T-cell lymphomas in mice. Pim1 is involved in the control of cytokine-mediated cell proliferation, differentiation and survival of lymphoid and myeloid cells as well as others (1). Expression of PIM1 can be stimulated by a variety of growth factors and is regulated at four different levels: transcriptional, post-transcriptional, translational and post-translational (2). Expression of Pim1 is mediated through activation of the JAK/STAT pathway. Pim1 is activated by phosphorylation at S280 and Y309 (by Etk). Pim1 has been linked with the development of prostate cancer, colorectal adenocarcinomas, large-cell lymphomas and hematopoietic malignancies.
References
[1] Meeker TC, Nagarajan L, ar-Rushdi A, Croce CM. Cloning and characterization of the human PIM-1 gene: a putative oncogene related to the protein kinases. J Cell Biochem. 1987 Oct;35(2):105-12. PMID:3429489
[2] Friedmann M, Nissen MS, Hoover DS, Reeves R, Magnuson NS. Characterization of the proto-oncogene pim-1: kinase activity and substrate recognition sequence. Arch Biochem Biophys. 1992 Nov 1;298(2):594-601. PMID: 1416988
[1] Meeker TC, Nagarajan L, ar-Rushdi A, Croce CM. Cloning and characterization of the human PIM-1 gene: a putative oncogene related to the protein kinases. J Cell Biochem. 1987 Oct;35(2):105-12. PMID:3429489
[2] Friedmann M, Nissen MS, Hoover DS, Reeves R, Magnuson NS. Characterization of the proto-oncogene pim-1: kinase activity and substrate recognition sequence. Arch Biochem Biophys. 1992 Nov 1;298(2):594-601. PMID: 1416988
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