Product Name: KinSub5DDDYV
Product Number: PE-01AKY95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub5DDDYV
Product Use: For assaying the phosphotransferase activity of BNDF/NT3/4/5 receptor- tyrosine kinase (TrkB, UniProt ID Q16620). The KinSub5DDDYV peptide demonstrated very high phosphotransferase activity with Blk, and exhibited high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub5DDDYV was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: FGGEDDDYVGVGGGG
Product Use: For assaying the phosphotransferase activity of BNDF/NT3/4/5 receptor- tyrosine kinase (TrkB, UniProt ID Q16620). The KinSub5DDDYV peptide demonstrated very high phosphotransferase activity with Blk, and exhibited high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub5DDDYV was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: FGGEDDDYVGVGGGG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1399.4 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Blk
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1399.4 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Blk
Scientific Background: TrkB is one of several protein kinases that can phosphorylate KinSub5DDDYV. Human TrkB is a receptor protein-tyrosine kinase of 822 amino acid length, with a predicted molecular mass of 91,999 Da. It is a member of the TK group of protein kinases in the Trk family. This kinase shows high variability in human tissue distribution with the highest levels detected in brain, ovary, pancreas, pituitary, spinal cord, thyroid and trachea. Orthologues of TrkB are highly conserved in vertebrates, including amphibians. TrkB is activated by binding BDNF, NT-3, NT-4 or NT-5, which induce dimerization and autophosphorylation (1, 2). Phosphorylation at Y516 increases phosphotransferase activity and induces interaction with PLCg1. TrkB activation stimulates signalling throught the MAPK pathway (2). Signalling through TrkB leads to cell differentiation. Mutations in the TrkB gene have been associated with obesity and mood disorders. TrkB has also been linked with the development of colon and lung cancers and neuroblastomas.
References
[1] Baxter GT, Radeke MJ, Kuo RC, Makrides V, Hinkle B, Hoang R, Medina-Selby A, Coit D, Valenzuela P, Feinstein SC. Signal transduction mediated by the truncated trkB receptor isoforms, trkB.T1 and trkB.T2. J Neurosci. 1997 Apr 15;17(8):2683-90. PMID: 9092589
[2] Pearse RN, Swendeman SL, Li Y, Rafii D, Hempstead BL. A neurotrophin axis in myeloma: TrkB and BDNF promote tumor-cell survival. Blood. 2005 Jun 1;105(11):4429-36. Epub 2005 Jan 18. PMID: 15657181
[1] Baxter GT, Radeke MJ, Kuo RC, Makrides V, Hinkle B, Hoang R, Medina-Selby A, Coit D, Valenzuela P, Feinstein SC. Signal transduction mediated by the truncated trkB receptor isoforms, trkB.T1 and trkB.T2. J Neurosci. 1997 Apr 15;17(8):2683-90. PMID: 9092589
[2] Pearse RN, Swendeman SL, Li Y, Rafii D, Hempstead BL. A neurotrophin axis in myeloma: TrkB and BDNF promote tumor-cell survival. Blood. 2005 Jun 1;105(11):4429-36. Epub 2005 Jan 18. PMID: 15657181
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