Product Name: KinSub4RRDSP
Product Number: PE-01AKU95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub4RRDSP
Product Use: For assaying the phosphotransferase activity of Cyclin-dependent protein-serine kinase 5 (CDK5, UniProt ID Q00535). The KinSub4RRDSP peptide demonstrated very high phosphotransferase activity with CHK2, and exhibited very low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub4RRDSP was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GFRGRRDSPRKKMHG
Product Use: For assaying the phosphotransferase activity of Cyclin-dependent protein-serine kinase 5 (CDK5, UniProt ID Q00535). The KinSub4RRDSP peptide demonstrated very high phosphotransferase activity with CHK2, and exhibited very low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub4RRDSP was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GFRGRRDSPRKKMHG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1784.1 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: CHK2
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1784.1 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: CHK2
Scientific Background: CDK5 is a protein-serine/threonine kinase of the CMGC group and CDK family. It is required for cell cycle arrest in neuronal cells. It is activated by binding p35 protein. Phosphorylation at S159 increases phosphotransferase activity. Phosphorylation at T14 and Y15 inhibits phosphotransferase activity. CDK5 has been observed to be proline-directed and to phosphorylate SRC, NOS3, VIM/vimentin, p35/CDK5R1, MEF2A, SIPA1L1, SH3GLB1, PXN, PAK1, MCAM/MUC18, SEPT5, SYN1, DNM1, AMPH, SYNJ1, CDK16, RAC1, RHOA, CDC42, TONEBP/NFAT5, MAPT/TAU, MAP1B, histone H1, p53/TP53, HDAC1, APEX1, PTK2/FAK1, huntingtin/HTT, ATM, MAP2, NEFH and NEFM. CDK5 has been implicated in Aneurysmal bone cyst, which is a bone cancer that will affect bone, endothelial tissue, and bone marrow.
References
[1] Tang D, Wang JH. Cyclin-dependent kinase 5 (Cdk5) and neuron-specific Cdk5 activators. Prog Cell Cycle Res. 1996;2:205-16. PMID: 9552397.
[2] Dhavan R, Tsai LH. A decade of CDK5. Nat Rev Mol Cell Biol. 2001 Oct;2(10):749-59. PMID: 11584302.
[1] Tang D, Wang JH. Cyclin-dependent kinase 5 (Cdk5) and neuron-specific Cdk5 activators. Prog Cell Cycle Res. 1996;2:205-16. PMID: 9552397.
[2] Dhavan R, Tsai LH. A decade of CDK5. Nat Rev Mol Cell Biol. 2001 Oct;2(10):749-59. PMID: 11584302.
© Kinexus Bioinformatics Corporation 2017