Product Name: KinSub3RRMSF
Product Number: PE-01AKR95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub3RRMSF
Product Use: For assaying the phosphotransferase activity of p21-activated kinase 4 (PAK4, UniProt ID O96013). The KinSub3RRMSF peptide demonstrated high phosphotransferase activity with PAK5 (PAK7), and exhibited very high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub3RRMSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGDGRRMSFDGCGGG
Product Use: For assaying the phosphotransferase activity of p21-activated kinase 4 (PAK4, UniProt ID O96013). The KinSub3RRMSF peptide demonstrated high phosphotransferase activity with PAK5 (PAK7), and exhibited very high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub3RRMSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGDGRRMSFDGCGGG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1427.6 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: PAK5 (PAK7)
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1427.6 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: PAK5 (PAK7)
Scientific Background: PAK4 is one of several protein kinases that can phosphorylate KinSub3RRMSF. Human PAK4 is a protein-serine/threonine kinase of 591 amino acid length, with a predicted molecular mass of 64,072 Da. It is a member of the STE group of protein kinases in the STE20 family, and PAKB subfamily. This kinase is highly expressed and widely distributed in most tested human tissues. Orthologues of PAK4 are highly conserved in vertebrates, including amphibians. PAK4 has been implicated in the regulation of cytoskeletal organization, and cell morphology, motility and transformation. Binding of GTP-bound Cdc42 to the autoregulatory region at the N-terminus of PAK4 releases monomers from the autoinhibited dimer, enables phosphorylation at S474 and activation of its phosphotransferase activity. PAK4 has been linked with the development of cancer.
References
[1] Jaffer ZM, Chernoff J. p21-activated kinases: three more join the Pak. Int J Biochem Cell Biol. 2002 Jul;34(7):713-7. PMID:11950587
[2] Qu J, Cammarano MS, Shi Q, Ha KC, de Lanerolle P, Minden A. Activated PAK4 regulates cell adhesion and anchorage-independent growth. Mol Cell Biol. 2001 May;21(10):3523-33. PMID: 11313478 PMCID: PMC100274
[1] Jaffer ZM, Chernoff J. p21-activated kinases: three more join the Pak. Int J Biochem Cell Biol. 2002 Jul;34(7):713-7. PMID:11950587
[2] Qu J, Cammarano MS, Shi Q, Ha KC, de Lanerolle P, Minden A. Activated PAK4 regulates cell adhesion and anchorage-independent growth. Mol Cell Biol. 2001 May;21(10):3523-33. PMID: 11313478 PMCID: PMC100274
© Kinexus Bioinformatics Corporation 2017