Product Name: KinSub3RRGSF
Product Number: PE-01AKP95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub3RRGSF
Product Use: For assaying the phosphotransferase activity of Protein kinase C epsilon type (PKCe, UniProt ID Q02156). The KinSub3RRGSF peptide demonstrated high phosphotransferase activity with TXK, and exhibited low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub3RRGSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GFLSRRGSFRGGGHG
Peptide Modifications N Terminus: Free amino
Product Use: For assaying the phosphotransferase activity of Protein kinase C epsilon type (PKCe, UniProt ID Q02156). The KinSub3RRGSF peptide demonstrated high phosphotransferase activity with TXK, and exhibited low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub3RRGSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GFLSRRGSFRGGGHG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1546.7 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: TXK
Peptide Molecular Mass Calculated: 1546.7 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: TXK
Scientific Background: PKCe is one of several protein kinases that can phosphorylate KinSub3RRGSF. Human PKCe is a protein-serine/threonine kinase of 737 amino acid length, with a predicted molecular mass of 83,674 Da. It is a member of the AGC group of protein kinases in the PKC family, and Eta subfamily. This kinase is moderate to highly expressed in most tested human tissues except in the brain and spinal cord where it is poorly expressed. Orthologues of PKCe are amongst the most highly conserved protein kinases in animals, plants, fungi and unicellular eukaryotes. PKCe is activated by phosphorylation at T566, T710 and S729. Phosphorylation of S729 also promotes dephosphorylation of T566 and possibly nuclear import. Phosphorylation at S368 induces interaction with 14-3-3 beta. PKCe is involved in many different cellular functions, such as neuron channel activation, cardioprotection from ischemia (1), heat shock response, as well as insulin exocytosis. Knockout studies in mice indicate that this kinase is important for lipopolysaccharide (LPS)-mediated signalling in activated macrophages and may control anxiety-like behavior (2). PKCe has been linked with the development of Alzheimer's disease, colorectal adenocarcinoma, prostate and thyroid cancers.
References
[1] Chen CH, Gray MO, Mochly-Rosen D. Cardioprotection from ischemia by a brief exposure to physiological levels of ethanol: role of epsilon protein kinase C. Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12784-9. PMID: 10536000 PMCID: PMC23099
[2] Hodge CW, Raber J, McMahon T, Walter H, Sanchez-Perez AM, Olive MF, Mehmert K, Morrow AL, Messing RO. Decreased anxiety-like behavior, reduced stress hormones, and neurosteroid supersensitivity in mice lacking protein kinase Cepsilon. J Clin Invest. 2002 Oct;110(7):1003-10. PMID:12370278 PMCID: PMC151152
[1] Chen CH, Gray MO, Mochly-Rosen D. Cardioprotection from ischemia by a brief exposure to physiological levels of ethanol: role of epsilon protein kinase C. Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12784-9. PMID: 10536000 PMCID: PMC23099
[2] Hodge CW, Raber J, McMahon T, Walter H, Sanchez-Perez AM, Olive MF, Mehmert K, Morrow AL, Messing RO. Decreased anxiety-like behavior, reduced stress hormones, and neurosteroid supersensitivity in mice lacking protein kinase Cepsilon. J Clin Invest. 2002 Oct;110(7):1003-10. PMID:12370278 PMCID: PMC151152
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