Product Name: KinSub3RRDSF
Product Number: PE-01AKN95
Size: 200 µg      Price:99.00
      $US
Peptide Name: KinSub3RRDSF

Product Use: For assaying the phosphotransferase activity of NIMA (never-in-mitosis)-related protein-serine kinase 7 (NEK7, UniProt ID Q8TDX7). The KinSub3RRDSF peptide demonstrated high phosphotransferase activity with Pim1, and exhibited very low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.

Peptide Production Method: Solid-phase peptide synthesis

Peptide Origin: KinSub3RRDSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.

Peptide Sequence: HGRSRRDSFRFRGHG

Peptide Modifications N Terminus: Free amino

Peptide Modifications C Terminus: Amide

Peptide Molecular Mass Calculated: 1827 Da

Peptide Purity Percent after Synthesis and Purification: >95

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Peptide Recommended Enzyme: Pim1

Scientific Background: Nek7 is one of several protein kinases that can phosphorylate KinSub3RRDSF. Human Nek7 is a protein-serine/threonine kinase of 302 amino acid length, with a predicted molecular mass of 34,551 Da. It is a member of the Other group of protein kinases in the Nek family. This kinase is highly expressed and widely distributed in most tested human tissues. Orthologues of Nek7 are highly conserved in vertebrates and insects. Nek7 is a member of the NIMA family of serine/threonine kinases. In contrast to the other documented NimA-related kinases, Nek7 harbor its catalytic domain in the C-terminus of the protein. Immunofluorescence studies indicate that Nek7 is cytoplasmic and located on chromosome 1 (1). The major protein kinase that is active on the p70 S6 kinase hydrophobic regulatory site (FXXFS/TF/Y) T412, was purified from rat liver and identified as Nek7 (2). Nek7 phosphotransferase activity is rapidly and efficiently increased by serum deprivation, and may be regulated in a cell cycle-dependent manner. Nek7 has been linked with the development of ovarian serous carcinomas.
References

[1] Kandli M, Feige E, Chen A, Kilfin G, Motro B. Isolation and characterization of two evolutionarily conserved murine kinases (Nek6 and nek7) related to the fungal mitotic regulator, NIMA. Genomics. 2000 Sep 1;68(2):187-96. PMID:10964517

[2] Belham C. Identification of the NIMA family kinases Nek6/7 as regulators of the p70 ribosomal S6 kinase. Comb MJ, Avruch J. Curr Biol. 2001 Aug 7;11(15):1155-67. PMID: 11516946