Product Name: KinSub3RPLSP
Product Number: PE-01AKM95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub3RPLSP
Product Use: For assaying the phosphotransferase activity of Protein-serine kinase C mu (Protein kinase D) (PKD1, UniProt ID Q15139). The KinSub3RPLSP peptide demonstrated medium phosphotransferase activity with Brk, and exhibited medium specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub3RPLSP was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRSRPLSPGKGGYG
Product Use: For assaying the phosphotransferase activity of Protein-serine kinase C mu (Protein kinase D) (PKD1, UniProt ID Q15139). The KinSub3RPLSP peptide demonstrated medium phosphotransferase activity with Brk, and exhibited medium specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub3RPLSP was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGRSRPLSPGKGGYG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1444.6 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Brk
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1444.6 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Brk
Scientific Background: PKD1 is one of several protein kinases that can phosphorylate KinSub3RPLSP. Human PKD1 (also known as PKC-mu) is a protein-serine/threonine kinase of 912 amino acid length, with a predicted molecular mass of 101,704 Da. It is a member of the CAMK group of protein kinases in the PKD family. It is moderate to highly expressed in most tested human tissues, especially in the thymus, lung and peripheral blood mononuclear cells (1), but poorly expressed in the brain and spinal cord. Orthologues of PKD1 are highly conserved in vertebrates, including amphibians. PKD1 is activated by DAG and phorbol esters, which mediates translocation to the cell membrane and trans-Golgi network. Phosphorylation at Y95 increases kinase activity and induces interaction with PKC-delta. Phosphorylation at S249, Y463, S738, S742 and S910 increases kinase activity. Phosphorylation at S738 and S742 also induces interaction with ASK1, JNK1 and IKKb. PKD1 forms a complex in vivo with a PI4-kinase and a PI4-phosphate 5-kinase. A region of PKD1 between the amino-terminal transmembrane domain and the pleckstrin homology domain is shown to be involved in the association with the lipid kinases (2). PKD1 has been linked with the development of colorectal adenocarcinomas, lung bronchoalveolar carcinomas and melanomas (metastatic).
References
[1] Rennecke J, Johannes FJ, Richter KH, Kittstein W, Marks F, Gschwendt M. Immunological demonstration of protein kinase C mu in murine tissues and various cell lines. Differential recognition of phosphorylated forms and lack of down-regulation upon 12-O-tetradecanoylphorphol-13-acetate treatment of cells. Eur J Biochem. 1996 Dec 1;242(2):428-32. PMID: 8973662
[2] Nishikawa K, Toker A, Wong K, Marignani PA, Johannes FJ, Cantley LC. Association of protein kinase Cmu with type II phosphatidylinositol 4-kinase and type I phosphatidylinositol-4-phosphate 5-kinase. J Biol Chem. 1998 Sep 4;273(36):23126-33. PMID: 9722541
[1] Rennecke J, Johannes FJ, Richter KH, Kittstein W, Marks F, Gschwendt M. Immunological demonstration of protein kinase C mu in murine tissues and various cell lines. Differential recognition of phosphorylated forms and lack of down-regulation upon 12-O-tetradecanoylphorphol-13-acetate treatment of cells. Eur J Biochem. 1996 Dec 1;242(2):428-32. PMID: 8973662
[2] Nishikawa K, Toker A, Wong K, Marignani PA, Johannes FJ, Cantley LC. Association of protein kinase Cmu with type II phosphatidylinositol 4-kinase and type I phosphatidylinositol-4-phosphate 5-kinase. J Biol Chem. 1998 Sep 4;273(36):23126-33. PMID: 9722541
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