Product Name: KinSub3DDDYF
Product Number: PE-01AKH95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub3DDDYF
Product Use: For assaying the phosphotransferase activity of Macrophage colony-stimulating factor 1 receptor (CSF1R, UniProt ID P07333). The KinSub3DDDYF peptide demonstrated very high phosphotransferase activity with Blk, and exhibited high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub3DDDYF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGREDDDYFGVGGGG
Product Use: For assaying the phosphotransferase activity of Macrophage colony-stimulating factor 1 receptor (CSF1R, UniProt ID P07333). The KinSub3DDDYF peptide demonstrated very high phosphotransferase activity with Blk, and exhibited high specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub3DDDYF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GGREDDDYFGVGGGG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1456.4 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Blk
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1456.4 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: Blk
Scientific Background: CSF1R is one of several protein kinases that can phosphorylate KinSub3DDDYF. Human CSF1R (also known as Fms) is a protein-tyrosine kinase of 972 amino acid length, with a predicted molecular mass of 107,984 Da. It is a member of the TK group of protein kinases in the PDGFR family. This kinase is highly expressed and widely distributed in most tested human tissues except in the brain and spinal cord, where it is more moderately expressed. Orthologues of CSF1R are highly conserved in mammals and birds. CSF1R contains a so-called kinase insert domain and is a member of the CSF1/PDGF receptor family of tyrosine-protein kinases. CSF1R is activated by binding of macrophage colony-stimulating factor 1 (CSF1), which induces dimerization and autophosphorylation. CSF1R mediates most if not all of the biological effects of CSF1 which control the production, differentiation, and function of cell of the monocyte/macrophage lineage (1). Mutations in CSF1R have been associated with providing sustained signals for cell growth and a predisposition to myeloid malignancy (2). CSF1R has been linked with the development of acute myeloid leukemia, chronic myelomonocytic leukemia, myelodysplasia, hepatocellular carcinomas, breast cancer, and lung squamous cell carcinomas (LSCC).
References
[1] Sherr CJ. Regulation of mononuclear phagocyte proliferation by colony-stimulating factor-1. Int J Cell Cloning. 1990 Jan;8 Suppl 1:46-60; discussion 60-2. PMID: 2157778
[2] Follows GA, Tagoh H, Richards SJ, Melnik S, Dickinson H, de Wynter E, Lefevre P, Morgan GJ, Bonifer C. c-FMS chromatin structure and expression in normal and leukaemic myelopoiesis. Oncogene. 2005 May 19;24(22):3643-51. PMID: 15806141
[1] Sherr CJ. Regulation of mononuclear phagocyte proliferation by colony-stimulating factor-1. Int J Cell Cloning. 1990 Jan;8 Suppl 1:46-60; discussion 60-2. PMID: 2157778
[2] Follows GA, Tagoh H, Richards SJ, Melnik S, Dickinson H, de Wynter E, Lefevre P, Morgan GJ, Bonifer C. c-FMS chromatin structure and expression in normal and leukaemic myelopoiesis. Oncogene. 2005 May 19;24(22):3643-51. PMID: 15806141
© Kinexus Bioinformatics Corporation 2017