Product Name: KinSub2RRLSF
Product Number: PE-01AKF95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub2RRLSF
Product Use: For assaying the phosphotransferase activity of calcium/calmodulin-dependent protein-serine kinase 4 (CaMK4, UniProt ID Q16566). The KinSub2RRLSF peptide demonstrated medium phosphotransferase activity with TXK, and exhibited moderate specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub2RRLSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GFLSRRLSFCNFKHG
Product Use: For assaying the phosphotransferase activity of calcium/calmodulin-dependent protein-serine kinase 4 (CaMK4, UniProt ID Q16566). The KinSub2RRLSF peptide demonstrated medium phosphotransferase activity with TXK, and exhibited moderate specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub2RRLSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GFLSRRLSFCNFKHG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1768.1 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: TXK
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1768.1 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: TXK
Scientific Background: CaMK4 is one of several protein kinases that can phosphorylate KinSub2RRLSF. Human CaMK4 is a protein-serine/threonine kinase of 473 amino acid length, with a predicted molecular mass of 51,926 Da. It is a member of the CAMK group of protein kinases in the CAMK1 family. This kinase is widely distributed from moderate to high levels in most human tissues with highest expression in lymph nodes, pancreas, spleen, testes, thymus and tonsils. CAMK4 is localized in neurons in the hippocampus, amygdala, anterior cingulate cortex, somatosensory cortex, and insular cortex (1). CAMK4 is involved in neural activity-dependent signalling in the neuronal nucleus and thought to plays an important role in the consolidation/retention of hippocampus-dependent long-term memory (2). Orthologues of CaMK4 are amongst the most highly conserved protein kinases in animals, plants, fungi and unicellular eukaryotes. CaMK4 is activated by Ca2+/calmodulin and phosphorylation at S12, S13 and T200 by CAMKK1 and CAMKK2. Autophosphorylation of S336 allows CaMK4 to switch to a Ca2+/calmodulin-independent state. Binding to PP2CA or PP2CB inhibits CaMK4 binding to Ca2+/calmodulin. CaMK4 has been linked with the development of lung adenocarcinomas and lung large cell carcinomas.
References
[1] Sikela JM, Law ML, Kao FT, Hartz JA, Wei Q, Hahn WE. Chromosomal localization of the human gene for brain Ca2+/calmodulin-dependent protein kinase type IV. Genomics. 1989 Jan;4(1):21-7. PMID: 2536634.
[2] Kang H, Sun LD, Atkins CM, Soderling TR, Wilson MA, Tonegawa S. An important role of neural activity-dependent CaMKIV signaling in the consolidation of long-term memory. Cell. 2001 Sep 21;106(6):771-83. PMID: 11572782.
[1] Sikela JM, Law ML, Kao FT, Hartz JA, Wei Q, Hahn WE. Chromosomal localization of the human gene for brain Ca2+/calmodulin-dependent protein kinase type IV. Genomics. 1989 Jan;4(1):21-7. PMID: 2536634.
[2] Kang H, Sun LD, Atkins CM, Soderling TR, Wilson MA, Tonegawa S. An important role of neural activity-dependent CaMKIV signaling in the consolidation of long-term memory. Cell. 2001 Sep 21;106(6):771-83. PMID: 11572782.
© Kinexus Bioinformatics Corporation 2017