Product Name: KinSub2RRKSF
Product Number: PE-01AKE95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub2RRKSF
Product Use: For assaying the phosphotransferase activity of Ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase 1 (p70S6K, UniProt ID P23443). The KinSub2RRKSF peptide demonstrated medium phosphotransferase activity with TXK, and exhibited low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub2RRKSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: HGGFRRKSFCGSGGY
Product Use: For assaying the phosphotransferase activity of Ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase 1 (p70S6K, UniProt ID P23443). The KinSub2RRKSF peptide demonstrated medium phosphotransferase activity with TXK, and exhibited low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub2RRKSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: HGGFRRKSFCGSGGY
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1614.8 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: TXK
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1614.8 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: TXK
Scientific Background: p70S6K is one of several protein kinases that can phosphorylate KinSub2RRKSF. Human p70S6K is a protein-serine/threonine kinase of 525 amino acid length, with a predicted molecular mass of 59,140 Da. It is a member of the AGC group of protein kinases in the RSK family, and p70 subfamily. This kinase is highly expressed and widely distributed in most tested human tissues except in the brain and spinal cord where it is more moderately expressed. Orthologues of p70S6K are highly conserved in animals and plants. p70S6K is activated by phosphorylation at T252 (by PDK1), S394 (by mTOR), T412 (by mTOR), S427, S434 (by ERK1/2), S441, T444 (by ERK1/2) and S447 (by ERK1/2). p70S6K is responsible for the phosphorylation of 40S ribosomal protein S6 (1). It is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6K phosphotransferase activity changes during the cell cycle, and increases 20-fold in G1 cells released from G0 (2). It has been linked with the development of diet-induced obesity, colon adenocarcinoma and breast cancer.
References
[1] Ferrari S, Thomas G. S6 phosphorylation and the p70s6k/p85s6k. Crit Rev Biochem Mol Biol. 1994;29(6):385-413. PMID: 7705100
[2] Edelmann HM, Kühne C, Petritsch C, Ballou LM. Cell cycle regulation of p70 S6 kinase and p42/p44 mitogen-activated protein kinases in Swiss mouse 3T3 fibroblasts. J Biol Chem. 1996 Jan 12;271(2):963-71. PMID: 8557712
[1] Ferrari S, Thomas G. S6 phosphorylation and the p70s6k/p85s6k. Crit Rev Biochem Mol Biol. 1994;29(6):385-413. PMID: 7705100
[2] Edelmann HM, Kühne C, Petritsch C, Ballou LM. Cell cycle regulation of p70 S6 kinase and p42/p44 mitogen-activated protein kinases in Swiss mouse 3T3 fibroblasts. J Biol Chem. 1996 Jan 12;271(2):963-71. PMID: 8557712
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