Product Name: KinSub1RRKSF
Product Number: PE-01AJF95
Size: 200 µg      Price:99.00
      $US
Peptide Name: KinSub1RRKSF

Product Use: For assaying the phosphotransferase activity of Tyrosine-protein kinase TXK (UniProt ID P42681). The KinSub1RRKSF peptide demonstrated moderate phosphotransferase activity with TXK, and exhibited low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.

Peptide Production Method: Solid-phase peptide synthesis

Peptide Origin: KinSub1RRKSF was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.

Peptide Sequence: HGGSRRKSFCGTTGY

Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide

Peptide Molecular Mass Calculated: 1612.8 Da

Peptide Purity Percent after Synthesis and Purification: >95

Peptide Appearance: White powder

Peptide Form: Solid

Storage Conditions: -20°C

Peptide Recommended Enzyme: TXK

Scientific Background: TXK is one of several protein kinases that can phosphorylate KinSub1RRKSF. Human TXK is a protein-tyrosine kinase of 527 amino acid length, with a predicted molecular mass of 61,258 Da. It is a member of the TK group of protein kinases in the Tec family. This kinase is moderate to highly expressed in most tested human tissues except in the brain, heart and spinal cord where it is poorly expressed. Orthologues of TXK are highly conserved in mammals. TXK or RLK is a member of the TEC family of non-receptor tyrosine kinases. TXK is expressed in T-cells and is an important component of signalling pathways downstream of lymphocyte antigen receptor. TXK is phosphorylated in response to T-cell receptor stimulation and can be activated by phosphorylation by Src family kinases. However, TXK is phosphorylated independent of PI-3K activity (1). Excessive TXK protein expression is seen in patients with Behcet's disease. Over production of TXK leads to increased Th1 cell function that is involved in the pathogenesis of Behcet's disease (2).
References

[1] Czar MJ, Debnath J, Schaeffer EM, Lewis CM, Schwartzberg PL. Biochemical and genetic analyses of the Tec kinases Itk and Rlk/Txk. Biochem Soc Trans. 2001 Nov;29(Pt 6):863-7. PMID: 11709089

[2] Suzuki N, Nara K, Suzuki T. Skewed Th1 responses caused by excessive expression of Txk, a member of the Tec family of tyrosine kinases, in patients with Behcet's disease. Clin Med Res. 2006 Jun;4(2):147-51. PMID:16809408 PMCID: PMC1483892