Product Name: KinSub1RRGSP
Product Number: PE-01AJD95
| Size: | 200 µg | Price: | 99.00 | |
| $US |
Peptide Name: KinSub1RRGSP
Product Use: For assaying the phosphotransferase activity of Dual specificity protein kinase CLK3 (UniProt ID P49761). The KinSub1RRGSP peptide demonstrated high phosphotransferase activity with TXK, and exhibited very low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RRGSP was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GFRGRRGSPGGGGYG
Product Use: For assaying the phosphotransferase activity of Dual specificity protein kinase CLK3 (UniProt ID P49761). The KinSub1RRGSP peptide demonstrated high phosphotransferase activity with TXK, and exhibited very low specificity when assayed with over 200 other protein kinases. A listing of other kinases that show appreciable phosphotransferase activity towards this peptide are listed in Table 1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: KinSub1RRGSP was originally identified using a microarray with peptides that were predicted as optimal substrates for 500 human protein kinases with a proprietary algorithm developed at Kinexus with our academic partners.
Peptide Sequence: GFRGRRGSPGGGGYG
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1436.6 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: TXK
Peptide Modifications C Terminus: Amide
Peptide Molecular Mass Calculated: 1436.6 Da
Peptide Purity Percent after Synthesis and Purification: >95
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C
Peptide Recommended Enzyme: TXK
Scientific Background: CLK3 is one of several protein kinases that can phosphorylate KinSub1RRGSP. Human CLK3 is a protein-serine/threonine kinase of 490 amino acid length, with a predicted molecular mass of 58,588 Da. It is a member of the CMGC group of protein kinases in the CLK family. This kinase is moderate to highly expressed in most tested human tissues except in the brain and spinal cord where it is poorly expressed. Orthologues of CLK3 are highly conserved in vertebrates, including amphibians. Long and short isoforms (phCLK3 and pCLK3/152) result from alternative splicing and coexist in different tissues (1). The CLK3 protein has the molecular functions of ATP binding, nucleotide binding, protein serine/threonine kinase activity, protein-tyrosine kinase activity and transferase activity and the CLK3 protein localizes in both the cytoplasm and nuclear compartments. CLK3 is thought to regulate the intranuclear distribution of the serine/arginine-rich (SR) family of splicing factors.
© Kinexus Bioinformatics Corporation 2017